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| 題名: | Identification of the chitinase genes from the diamondback moth, Plutella xylostella |
| 作者: | 高永旭;Liao, Z.H.;Kuo, T.C.;Kao, C.H.;Chou, T.M.;Kao, Y.H.;Huang, R.N. |
| 貢獻者: | 生醫理工學院生命科學系 |
| 關鍵詞: | active sites;amino acid sequences;Amino acids;Animals;Brassicaceae;Butterflies & moths;Catalytic Domain;Chitin;Chitin - metabolism;chitinase;Chitinases - chemistry;Chitinases - genetics;complementary DNA;Genes;Metabolism;moths;Moths - genetics;pests;Phylogeny;Plutella xylostella;Polymerase Chain Reaction;Research Papers;Sequence Alignment;Sequence Analysis, Protein;transcriptomics |
| 日期: | 2016-12-01 |
| 上傳時間: | 2026-04-23 11:13:03 (UTC+8) |
| 出版者: | Cambridge University Press;Cambridge, UK: Cambridge University Press |
| 摘要: | 摘要： Chitinases have an indispensable function in chitin metabolism and are well characterized in numerous insect species. Although the diamondback moth (DBM) Plutella xylostella, which has a high reproductive potential, short generation time, and characteristic adaptation to adverse environments, has become one of the most serious pests of cruciferous plants worldwide, the information on the chitinases of the moth is presently limited. In the present study, using degenerated polymerase chain reaction (PCR) and rapid amplification of cDNA ends-PCR strategies, four chitinase genes of P. xylostella were cloned, and an exhaustive search was conducted for chitinase-like sequences from the P. xylostella genome and transcriptomic database. Based on the domain analysis of the deduced amino acid sequences and the phylogenetic analysis of the catalytic domain sequences, we identified 15 chitinase genes from P. xylostella. Two of the gut-specific chitinases did not cluster with any of the known phylogenetic groups of chitinases and might be in a new group of the chitinase family. Moreover, in our study, group VIII chitinase was not identified. The structures, classifications and expression patterns of the chitinases of P. xylostella were further delineated, and with this information, further investigations on the functions of chitinase genes in DBM could be facilitated.
其他題名： Bull. Entomol. Res
出版者： Cambridge, UK: Cambridge University Press
出版日期： 2016-12
出處： Bulletin of entomological research, 2016-12, Vol.106 (6), p.769-780
資源來源： Agricultural & Environmental Science Collection
版權： Copyright © Cambridge University Press 2016
識別號： ISSN: 0007-4853
識別號： ISSN: 1475-2670
識別號： EISSN: 1475-2670
識別號： DOI: 10.1017/S0007485316000511
識別號： PMID: 27417424 |
| 顯示於類別: | [生命科學系] 期刊論文
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