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| 題名: | Regulation of Dense-Core Granule Replenishment by Autocrine BMP Signalling in Drosophila Secondary Cells |
| 作者: | 范世榮;Redhai, Siamak;Hellberg, Josephine E. E. U.;Wainwright, Mark;Perera, Sumeth W.;Castellanos, Felix;Kroeger, Benjamin;Gandy, Carina;Leiblich, Aaron;Corrigan, Laura;Hilton, Thomas;Patel, Benjamin;Fan, Shih-Jung;Hamdy, Freddie;Goberdhan, Deborah C. I.;Wilson, Clive |
| 貢獻者: | 生醫理工學院生命科學系 |
| 關鍵詞: | Anatomy & physiology;Animals;Autocrine Communication - genetics;Biology and life sciences;Biosynthesis;Bone morphogenetic proteins;Bone Morphogenetic Proteins - genetics;Cellular signal transduction;Colleges & universities;Drosophila;Drosophila melanogaster;Drosophila melanogaster - genetics;Drosophila melanogaster - growth & development;Drosophila Proteins - biosynthesis;Drosophila Proteins - genetics;Enzymes;Funding;Gene expression;Gene Expression Regulation, Developmental - genetics;Genetic aspects;Health aspects;Hospitals;Insects;Male;Males;Medical research;Medicine and Health Sciences;Neurons - metabolism;Physiology;Prostate;Prostate - growth & development;Prostate - metabolism;Proteins;Research and analysis methods;Secretory Vesicles - genetics;Secretory Vesicles - metabolism;Sexual Behavior, Animal - physiology;Signal Transduction - genetics |
| 日期: | 2016-10-01 |
| 上傳時間: | 2026-04-23 11:13:43 (UTC+8) |
| 出版者: | Public Library of Science;United States: Public Library of Science |
| 摘要: | 摘要： Regulated secretion by glands and neurons involves release of signalling molecules and enzymes selectively concentrated in dense-core granules (DCGs). Although we understand how many secretagogues stimulate DCG release, how DCG biogenesis is then accelerated to replenish the DCG pool remains poorly characterised. Here we demonstrate that each prostate-like secondary cell (SC) in the paired adult Drosophila melanogaster male accessory glands contains approximately ten large DCGs, which are loaded with the Bone Morphogenetic Protein (BMP) ligand Decapentaplegic (Dpp). These DCGs can be marked in living tissue by a glycophosphatidylinositol (GPI) lipid-anchored form of GFP. In virgin males, BMP signalling is sporadically activated by constitutive DCG secretion. Upon mating, approximately four DCGs are typically released immediately, increasing BMP signalling, primarily via an autocrine mechanism. Using inducible knockdown specifically in adult SCs, we show that secretion requires the Soluble NSF Attachment Protein, SNAP24. Furthermore, mating-dependent BMP signalling not only promotes cell growth, but is also necessary to accelerate biogenesis of new DCGs, restoring DCG number within 24 h. Our analysis therefore reveals an autocrine BMP-mediated feedback mechanism for matching DCG release to replenishment as secretion rates fluctuate, and might explain why in other disease-relevant systems, like pancreatic β-cells, BMP signalling is also implicated in the control of secretion.
其他題名： PLoS Genet
出版者： United States: Public Library of Science
出版日期： 2016-10-11
出處： PLoS genetics, 2016-10, Vol.12 (10), p.e1006366-e1006366
資源來源： EBSCOhost OmniFile Full Text Select
版權： COPYRIGHT 2016 Public Library of Science
版權： 2016 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Secondary Cells. PLoS Genet 12(10): e1006366. doi:10.1371/journal.pgen.1006366
版權： 2016 Redhai et al 2016 Redhai et al
識別號： ISSN: 1553-7404
識別號： ISSN: 1553-7390
識別號： EISSN: 1553-7404
識別號： DOI: 10.1371/journal.pgen.1006366
識別號： PMID: 27727275 |
| 顯示於類別: | [生命科學系] 期刊論文
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