Vanderwaltozyma polyspora possesses two glycyl-tRNA synthetase genes: One constitutive and one inducible

NCU Institutional Repository > 生醫理工學院 > 生命科學系 > 期刊論文 > Item 987654321/102653

請使用永久網址來引用或連結此文件: https://ir.lib.ncu.edu.tw/handle/987654321/102653

題名:	Vanderwaltozyma polyspora possesses two glycyl-tRNA synthetase genes: One constitutive and one inducible
作者:	陳青諭;Chien, Chin-I;Chen, Yueh-Lin;Chen, Shun-Jia;Chou, Chi-Mao;Chen, Chin-Yu;Wang, Chien-Chia
貢獻者:	生醫理工學院生命科學系
關鍵詞:	Amino Acid Sequence;Aminoacyl-tRNA synthetase;Ascomycota - classification;Ascomycota - enzymology;Ascomycota - genetics;Ascomycota - physiology;Base Sequence;Constitutive gene;Dual function;ethanol;genes;glycine-tRNA ligase;Glycine-tRNA Ligase - chemistry;Glycine-tRNA Ligase - genetics;Glycine-tRNA Ligase - metabolism;Heat-Shock Response;Inducible gene;mitochondria;Molecular Sequence Data;promoter regions;protein synthesis;Saccharomyces cerevisiae;Stress;temperature;transfer RNA;yeasts
日期:	2015-03-01
上傳時間:	2026-04-23 11:14:19 (UTC+8)
出版者:	Academic Press Inc.;United States: Elsevier Inc
摘要:	摘要： •Vanderwaltozyma polyspora possesses two paralogous GlyRS genes—GRS1 and GRS2.•GRS1 provides functions in both the cytoplasm and mitochondria.•GRS2 is normally silent, but can be activated by stresses. Aminoacyl-tRNA synthetases are housekeeping enzymes essential for protein synthesis. We herein present evidence that the yeast Vanderwaltozyma polyspora possesses two paralogous glycyl-tRNA synthetase (GlyRS) genes—GRS1 and GRS2. Paradoxically, GRS1 provided functions in both the cytoplasm and mitochondria, while GRS2 was essentially silent under normal growth conditions. Expression of GRS2 could be activated by stresses such as high pH or ethanol and most effectively by high temperature. The expressed GlyRS2 protein was exclusively found in the cytoplasm and more stable under heat-shock conditions (37°C) than under normal growth conditions (30°C) in vivo. In addition, GRS2 effectively rescued the cytoplasmic defect of a Saccharomyces cerevisiae GRS1 knockout strain when expressed from a constitutive promoter. Moreover, the purified GlyRS2 enzyme was fairly active at both 30°C and 37°C in glycylation of yeast tRNA in vitro. However, unexpectedly, the purified GlyRS2 enzyme was practically inactive at temperature above 40°C in vitro. Our study suggests that GRS2 is an inducible gene that acts under stress conditions where GlyRS1 may be insufficient, unavailable, or rendered inactive. 其他題名： Fungal Genet Biol 出版者： United States: Elsevier Inc 出版日期： 2015-03 出處： Fungal genetics and biology, 2015-03, Vol.76, p.47-56 版權： 2015 Elsevier Inc. 版權： Copyright © 2015 Elsevier Inc. All rights reserved. 識別號： ISSN: 1087-1845 識別號： ISSN: 1096-0937 識別號： EISSN: 1096-0937 識別號： DOI: 10.1016/j.fgb.2015.02.004 識別號： PMID: 25683380
顯示於類別:	[生命科學系] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html		0Kb	HTML	20	檢視/開啟

在NCUIR中所有的資料項目都受到原著作權保護.

社群 sharing

資料載入中.....