Mary Ann Liebert Inc.;Los Angeles, CA: SAGE Publications
摘要:
摘要： Background: Urothelial carcinoma (UC) is the most common histologic subtype of bladder cancer. The administration of mitomycin C (MMC) into the bladder after transurethral resection of the bladder tumor (TURBT) is a common treatment strategy for preventing recurrence after surgery. We previously applied hydrostatic pressure combined with MMC in UC cells and found that hydrostatic pressure synergistically enhanced MMC-induced UC cell apoptosis through the Fas/FasL pathways. To understand the alteration of gene expressions in UC cells caused by hydrostatic pressure and MMC, oligonucleotide microarray was used to explore all the differentially expressed genes. Results: After bioinformatics analysis and gene annotation, Toll-like receptor 6 (TLR6) and connective tissue growth factor (CTGF) showed significant upregulation among altered genes, and their gene and protein expressions with each treatment of UC cells were validated by quantitative real-time PCR and immunoblotting. Conclusion: Under treatment with MMC and hydrostatic pressure, UC cells showed increasing apoptosis using extrinsic pathways through upregulation of TLR6 and CTGF. 其他題名： Genet Test Mol Biomarkers 出版者： Los Angeles, CA: SAGE Publications 出版日期： 2014-06-01 出處： Genetic testing and molecular biomarkers, 2014-06, Vol.18 (6), p.410-416 版權： Copyright 2014, Mary Ann Liebert, Inc. 識別號： ISSN: 1945-0265 識別號： ISSN: 1945-0257 識別號： EISSN: 1945-0257 識別號： DOI: 10.1089/gtmb.2013.0443 識別號： PMID: 24689870
