Comparative proteomic analysis of peritoneal dialysate from chronic glomerulonephritis patients

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題名:	Comparative proteomic analysis of peritoneal dialysate from chronic glomerulonephritis patients
作者:	吳忻怡;Wang, Hsien-Yi;Chien, Chih-Chiang;Liao, Pao-Chi;Huang, Chien-Cheng;Liao, Alex Chien Hwa;Wu, Hsin-Yi;Kan, Wei-Chih
貢獻者:	總教學中心通識教育中心
關鍵詞:	Apolipoproteins;Atherosclerosis;Biomarkers;Blotting, Western;Chromatography, Liquid;Dialysis;Dialysis Solutions - metabolism;Electrophoresis, Gel, Two-Dimensional;Glomerulonephritis - metabolism;Humans;Mass spectrometry;Medical technology;Patients;Peritoneal Dialysis;Protein Interaction Maps;Proteins;Proteome - metabolism;Proteomics - methods;Reproducibility of Results;Science;Tandem Mass Spectrometry
日期:	2013-06-12
上傳時間:	2026-04-23 12:52:30 (UTC+8)
出版者:	Hindawi Publishing Corporation;Cairo, Egypt: Hindawi Publishing Corporation
摘要:	摘要： Peritoneal dialysis (PD) frequently contributes to peritoneal damage which cannot be easily identified without invasive techniques, implying the urgent need for biomarkers and revealing mechanisms. Chronic glomerulonephritis (CGN) is one of the leading causes of receiving dialysis treatment. Here, we attempted to analyze the peritoneal dialysate collected from CGN patients when they receive continuous ambulatory peritoneal dialysis (CAPD) treatment for the first time and after a year to reveal the protein changes that resulted from PD. Proteins were displayed by two-dimensional gel electrophoresis (2DE). Altered gel spots were digested followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for protein identification. Eight proteins were found to have differential expression levels between two groups. Their differential expressions were validated by Western blots in other sets of peritoneal dialysates. Proteins identified with higher levels in the first-time dialysate suggested their dominant appearance in CGN patients, while those that showed higher levels in peritoneal dialysate collected after one year may result from initial peritoneal inflammation or changes in the permeability of the peritoneum to middle-sized proteins. All the identified proteins may provide a perceptiveness of peritoneal changes caused by PD and may function as potential biomarkers or drug targets. 其他題名： Biomed Res Int 出版者： Cairo, Egypt: Hindawi Publishing Corporation 出版日期： 2013-01-01 出處： BioMed research international, 2013-01, Vol.2013 (2013), p.1-10 資源來源： EBSCOhost Academic Search Premier 版權： Copyright © 2013 Hsin-Yi Wu et al. 版權： Copyright © 2013 Hsin-Yi Wu et al. Hsin-Yi Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 版權： Copyright © 2013 Hsin-Yi Wu et al. 2013 識別號： ISSN: 2314-6133 識別號： ISSN: 2314-6141 識別號： EISSN: 2314-6141 識別號： DOI: 10.1155/2013/863860 識別號： PMID: 23762862
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