Phosphorylation of CPAP by Aurora-A Maintains Spindle Pole Integrity during Mitosis

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題名:	Phosphorylation of CPAP by Aurora-A Maintains Spindle Pole Integrity during Mitosis
作者:	吳忻怡;Chou, En-Ju;Hung, Liang-Yi;Tang, Chieh-Ju C.;Hsu, Wen-Bin;Wu, Hsin-Yi;Liao, Pao-Chi;Tang, Tang K.
貢獻者:	總教學中心通識教育中心
關鍵詞:	Antigens - genetics;Antigens - metabolism;Aurora Kinase A - genetics;Aurora Kinase A - metabolism;Centrosome - physiology;HeLa Cells;Humans;Immunoprecipitation;Intracellular Signaling Peptides and Proteins - genetics;Intracellular Signaling Peptides and Proteins - metabolism;Microscopy, Confocal;Microtubule-Associated Proteins - antagonists & inhibitors;Microtubule-Associated Proteins - genetics;Microtubule-Associated Proteins - metabolism;Mitosis;Nerve Tissue Proteins - genetics;Nerve Tissue Proteins - metabolism;Phosphorylation;RNA Interference;RNA, Small Interfering - metabolism;Spindle Poles - physiology;Time-Lapse Imaging;Tubulin - genetics;Tubulin - metabolism
日期:	2016-03-29
上傳時間:	2026-04-23 12:55:12 (UTC+8)
出版者:	Cell Press;United States: Elsevier Inc
摘要:	摘要： CPAP is required for centriole elongation during S/G2 phase, but the role of CPAP in mitosis is incompletely understood. Here, we show that CPAP maintains spindle pole integrity through its phosphorylation by Aurora-A during mitosis. Depletion of CPAP induced a prolonged delay in mitosis, pericentriolar material (PCM) dispersion, and multiple mitotic abnormalities. Further studies demonstrated that CPAP directly interacts with and is phosphorylated by Aurora-A at serine 467 during mitosis. Interestingly, the dispersal of the PCM was effectively rescued by ectopic expression of wild-type CPAP or a phospho-mimic CPAP-S467D mutant, but not a non-phosphorylated CPAP-S467A mutant. Finally, we found that CPAP-S467D has a low affinity for microtubule binding but a high affinity for PCM proteins. Together, our results support a model wherein CPAP is required for proper mitotic progression, and phosphorylation of CPAP by Aurora-A is essential for maintaining spindle pole integrity. [Display omitted] •Depletion of CPAP induces multiple mitotic abnormalities•CPAP is phosphorylated by Aurora-A at Ser 467 during mitosis•Phosphorylated CPAP coheres PCM proteins and maintains centrosome integrity•Phosphorylated CPAP has a high affinity for PCM proteins but a low affinity for MTs Chou et al. show that CPAP is essential for proper mitotic progression and maintenance of spindle pole integrity. CPAP is phosphorylated by Aurora-A during mitosis and phosphorylated CPAP coheres PCM proteins and maintains centrosome integrity. 其他題名： Cell Rep 出版者： United States: Elsevier Inc 出版日期： 2016-03-29 出處： Cell Reports, 2016-03, Vol.14 (12), p.2975-2987 資源來源： Directory of Open Access Journals 版權： 2016 The Authors 版權： Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved. 識別號： ISSN: 2211-1247 識別號： ISSN: 2639-1856 識別號： EISSN: 2211-1247 識別號： DOI: 10.1016/j.celrep.2016.02.085 識別號： PMID: 26997271
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