Modulating the structure and function of an aminoacyl-trna synthetase cofactor by biotinylation

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題名:	Modulating the structure and function of an aminoacyl-trna synthetase cofactor by biotinylation
作者:	曾議寬;Chang, Chih-Yao;Chang, Chia-Pei;Chakraborty, Shruti;Wang, Shao-Win;Tseng, Yi-Kuan;Wang, Chien-Chia
貢獻者:	理學院統計研究所
關鍵詞:	aminoacyl tRNA synthetase;biotin;Biotinylation;Glutamate-tRNA Ligase - chemistry;Glutamate-tRNA Ligase - genetics;Glutamate-tRNA Ligase - metabolism;Hot Temperature;Methionine-tRNA Ligase - chemistry;Methionine-tRNA Ligase - genetics;Methionine-tRNA Ligase - metabolism;post-translational modification (PTM);Protein Stability;protein synthesis;Protein Synthesis and Degradation;RNA-Binding Proteins - chemistry;RNA-Binding Proteins - genetics;RNA-Binding Proteins - metabolism;Saccharomyces cerevisiae - chemistry;Saccharomyces cerevisiae - genetics;Saccharomyces cerevisiae - metabolism;Saccharomyces cerevisiae Proteins - chemistry;Saccharomyces cerevisiae Proteins - genetics;Saccharomyces cerevisiae Proteins - metabolism;transfer RNA (tRNA)
日期:	2016-08-12
上傳時間:	2026-04-23 12:56:34 (UTC+8)
出版者:	American Society for Biochemistry and Molecular Biology Inc.;United States: Elsevier Inc
摘要:	摘要： Arc1p is a yeast-specific tRNA-binding protein that forms a ternary complex with glutamyl-tRNA synthetase (GluRSc) and methionyl-tRNA synthetase (MetRS) in the cytoplasm to regulate their catalytic activities and subcellular distributions. Despite Arc1p not being involved in any known biotin-dependent reaction, it is a natural target of biotin modification. Results presented herein show that biotin modification had no obvious effect on the growth-supporting activity, subcellular distribution, tRNA binding, or interactions of Arc1p with GluRSc and MetRS. Nevertheless, biotinylation of Arc1p was temperature dependent; raising the growth temperature from 30 to 37 °C drastically reduced its biotinylation level. As a result, Arc1p purified from a yeast culture that had been grown overnight at 37 °C was essentially biotin free. Non-biotinylated Arc1p was more heat stable, more flexible in structure, and more effective than its biotinylated counterpart in promoting glutamylation activity of the otherwise inactive GluRSc at 37 °C in vitro. Our study suggests that the structure and function of Arc1p can be modulated via biotinylation in response to temperature changes. 其他題名： J Biol Chem 出版者： United States: Elsevier Inc 出版日期： 2016-08-12 出處： Journal of Biological Chemistry, 2016-08, Vol.291 (33), p.17102-17111 資源來源： Alma/SFX Local Collection 版權： 2016 © 2016 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. 版權： 2016 by The American Society for Biochemistry and Molecular Biology, Inc. 版權： 2016 by The American Society for Biochemistry and Molecular Biology, Inc. 2016 The American Society for Biochemistry and Molecular Biology, Inc. 識別號： ISSN: 0021-9258 識別號： ISSN: 1067-8816 識別號： ISSN: 1083-351X 識別號： EISSN: 1083-351X 識別號： DOI: 10.1074/jbc.M116.734343 識別號： PMID: 27330079
顯示於類別:	[統計研究所] 期刊論文

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