三團聯聚合物(PF-127)和磷脂質間交互作用力量測和微脂粒穩定度之探討; The study of interaction of PEO-PPO-PEO copolymer (PF-127) with phospholipid and comparison of physical stability of liposomes

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Title:	三團聯聚合物(PF-127)和磷脂質間交互作用力量測和微脂粒穩定度之探討;The study of interaction of PEO-PPO-PEO copolymer (PF-127) with phospholipid and comparison of physical stability of liposomes
Authors:	何怡瑱;Yi- Chan Ho
Contributors:	化學工程與材料工程研究所
Keywords:	界面;磷脂質;單分子層;微脂粒;interface;phospholipid;monolayer;liposome
Date:	2001-06-21
Issue Date:	2009-09-21 12:19:35 (UTC+8)
Publisher:	國立中央大學圖書館
Abstract:	本研究主要探討25 下，DMPC/PF-127（重量比為34：4、34：14和34：34）之Gibbs’吸附單分子層和PF-127分子吸附於三種不同單分子層（DMPC、DMPC/Vit-E及DMPC/cholesterol）的氣/液界面行為。首先就DMPC/PF-127 Gibbs’吸附單分子層系統而言，主要進行平衡表面張力量測和表面壓-每分子佔據面積等溫線（Π-A isotherm）的實驗。在所得的結果中，平衡表面張力隨著 PF-127添加量的增加呈現正比的線性關係。配合Gibbs表面過剩濃度關係式，可得表面過剩濃度和分子佔據面積。混合單分子層表面壓-每分子佔據面積的量測中，隨著PF-127添加量的增加，等溫線向右平移且有相變區（liquid-expanded transition liquid-condensed）的發生。 PF-127分子吸附在DMPC、DMPC/Vit-E、DMPC/ cholesterol三種不同單分子層的行為，則是藉由量測表面壓隨時間的變化的量測和表面壓-每分子佔據面積等溫線來進行研究。PF-127吸附在DMPC單分子層系統中，不同起始表面壓之表面壓隨時間變化的結果顯示，平衡表面壓與PF-127添加量呈正比關係。對於DMPC/Vit-E和DMPC/cholesterol混合單分子層系統，平衡表面壓的範圍分別是從 15.8∼28.3mN/m和 20.3~27.7mN/m，顯示添加維他命E和膽固醇改變分子間排列距離，使PF-127吸附於單分子層的表面壓產生差異。PF-127吸附平衡後之Π-A等溫線的結果顯示：（1）等溫線中有相變區的發生；（2）曲線向右平移，表示PF-127分子可能吸附或嵌入單分子層中，但因吸附的不夠緊或嵌入的不深，在高表面壓時，PF-127易被擠出單分子層中。此與微脂粒DSC實驗中，無論是嵌入法或吸附法都不影響相轉移溫度，推測PF-127嵌入脂質雙層不深是相符的。 This study investigated the behavior of mixed DMPC/PF-127 Gibbs’ adsorption monolayers (the weight ratio are 34: 4 , 34:14 and 34:34)and the PF-127 absorbed on different monolayers(the composition are DMPC, DMPC/Vit-E and DMPC/cholesterol)at the air/liquid interface at 25 . For mixed DMPC/PF-127 adsorption monolayers system at the air/water interface, the measurements of equilibrium surface tensions and surface pressure-area per molecule(Π-A) isotherms were carried out. The results of the mixed monolayer, the equilibrium surface tensions increased with the PF-127 concentration. But the excess of surface concentration is non- linear. TheΠ-A isotherms of the behavior of mixed DMPC/PF-127 monolayers indicate that the addition of the PF-127increased the shift percentages and the transition phases were obviously. The behavior of the PF-127 adsorbed on different monolayers were investigated from the measurements of the change of surface pressure with time andΠ-A isotherms. In the PF-127 adsorption of monolayer systems, the equilibrium adsorption surface pressure increased with the PF-127 concentration and the initial surface pressure. The behavior of PF-127 adsorbed on DMPC/Vit-E and DMPC/cholesterol mixed monolayers, the ranges of equilibrium adsorption surface pressures are 15.8~28.3mN/m and 20.3~27.7mN/m, indicated that the addition of α-tocopherol and cholesterol change the distance of mixed monolayers. TheΠ-A isotherms of the PF-127 adsorbed to balance indicate that the transition phases were carried out and the PF-127 might be insert or absorb to monolayers. However, at higher surface pressure, the PF-127 molecules are squeezed into the subphase. From the DSC (differential Scanning Calorimetry ) experiments of liposome, the change of temperatures of phase transition regardless of the PF-127 incorporated or adsorbed to liposomes, which suggest the PF-127 does not insert or absorb deeply. Finally, the results obtained in this study support that the PF-127 can be readily absorbed or inserted onto liposome, providing steric barrier , preventing the fusion or aggregation of liposomes.
Appears in Collections:	[National Central University Department of Chemical & Materials Engineering] Electronic Thesis & Dissertation

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