Deuterium NMR Study of the Effect of Ergosterol on POPE Membranes

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题名:	Deuterium NMR Study of the Effect of Ergosterol on POPE Membranes
作者:	Hsueh,YW;Weng,CJ;Chen,MT;Thewalt,J;Zuckermann,M
贡献者:	物理學系
关键词:	NUCLEAR-MAGNETIC-RESONANCE;ORIENTATIONAL ORDER PROFILE;SOLID-STATE NMR;LIPID-BILAYERS;DIPALMITOYLPHOSPHATIDYLCHOLINE MIXTURES;UNSATURATED PHOSPHATIDYLETHANOLAMINES;MOLECULAR-ORGANIZATION;MAXIMUM SOLUBILITY;PLASMA-MEMBRANE;PHASE-DIAGRAM
日期:	2010
上传时间:	2012-06-11 10:42:22 (UTC+8)
出版者:	國立中央大學
摘要:	We study the effect of ergosterol on the physical properties of 1-[(2)H(31)]palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) multibilayers using deuterium nuclear magnetic resonance. NMR spectra were taken as a function of temperature and ergosterol concentration up to 70 mol %. The spectral first moments show that there is a dramatic difference in the ability of ergosterol to disorder the gel phase and to order the liquid-crystalline phase of POPE membranes, an unusual behavior among lipid/sterol systems studied up to now. Further investigation of the liquid-crystalline phase shows that ergosterol (erg) increases the chain order of POPE-d31, but that this effect saturates at 10 mol % ergosterol. This is in marked contrast to the effect of cholesterol (chol) on POPE membranes: the chain order of POPE increases with cholesterol to at least 45 mol %. Moreover, we found that at higher ergosterol concentrations (>40 mol %) ergosterol decreases the POPE-d31 chain order, which, to our knowledge, has not been directly observed in other lipid/sterol systems. The temperature-composition phase diagram is presented. Finally, at all ergosterol concentrations, the chain order of liquid-crystalline-phase POPE is much smaller than that of comparable POPE/chol membranes. This implies that there is no liquid-ordered phase behavior for POPE/erg membranes.
關聯:	BIOPHYSICAL JOURNAL
显示于类别:	[物理學系] 期刊論文

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