為了精確調控生物材料表面性質以利於組織工程的應用，我們研發了利用電紡絲技術製備幾丁聚醣/褐藻酸鈉之複合纖維。首先，先進行純幾丁聚醣與純褐藻酸鈉的電紡絲研究。聚氧化乙烯(PEO)分別摻入於幾丁聚醣與褐藻酸鈉溶液中，並藉由掃描式電子顯微鏡(SEM)研究高分子組成對於纖維型態的影響。利用高分子溶液的黏度分析證實了高濃度的高分子造成的高黏度，影響纖維絲結構的型態。之後我們研究進料流率與纖維沉積的關係，藉由控制幾丁聚醣/聚氧化乙烯和褐藻酸鈉/聚氧化乙烯溶液的進料流率，可得到不同沉積比的奈米纖維。最後，所合成的複合奈米纖維被應用於細胞培養，因細胞在幾丁聚醣與褐藻酸鈉纖維絲的型態不同，我們可以透過奈米纖維的比例調控細胞的型態。在生物相容性上，發現幾丁聚醣與褐藻酸鈉的奈米纖維可以改善膜的細胞活性，此外複合纖維甚至比幾丁聚醣與褐藻酸鈉纖維絲有更好的生物相容性。這些結果表示，奈米纖維的比例可以透過同時紡絲技術加以控制，我們能夠操控複合材料的表面特性，這應有利於在生物材料的應用。To custom-tailor biomaterial surfaces for tissue engineering application, we developed chitosan/alginate composite surfaces using electrospinning technique. Firstly, chitosan and alginte were solely electrospun, respectively. Poly (ethylene oxide) (PEO) was added to both chitosan and alginte solutions, and the effect of polymer composition on fibrous morphology was studied through scanning electrical microscopy (SEM). In addition, viscosities of polymer solution were also analyzed, demonstrating that higher concentrations of polymer caused higher viscosities, which directly determined the morphology of nanofibrous structure. Then, the relationship between flow rates and fiber deposition was also studied. By controlling the flow rates of chitosan/PEO and alginate/PEO solution separately, these nanofibers were able to be spun simultaneously with different deposition ratios. Finally, the composite nanofibrous surfaces were applied for cell culture. Because cell morphologies were different in chitosan and alginate nanofibrous surfaces, we may regulate cell morphologies by controlling the ratios of nanofibers, About biocompatibility, it was found that nanofibers can improve the cell viability over chitosan and alginate films. In addition, composite nanofibers even demonstrated greater biocompatibility than that of chitosan and alginate fibers. These results suggested that the ratio of nanofiber can be finely controlled through simultaneous electrospinning technique, and we may manipulate composite surface properties, which should be beneficial to biomaterial application.
