探討培養溫度對巴西蘑菇的常溫/高溫菌株生產β-D-glucan之影響

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Title:	探討培養溫度對巴西蘑菇的常溫/高溫菌株生產β-D-glucan之影響
Authors:	葉怡芬;Yeh, Yi-fen
Contributors:	化學工程與材料工程學系
Keywords:	巴西蘑菇;液態發酵
Date:	2017-08-10
Issue Date:	2017-10-27 12:59:02 (UTC+8)
Publisher:	國立中央大學
Abstract:	食藥用菇的應用在東方文化已有幾千多年的歷史，自古以來在中藥典中即作為藥物以及菜餚的應用。巴西蘑菇學名Agaricus blazei Murill，又名巴西洋菇或姬松茸等，原產於中南美洲的巴西、祕魯地區，主要分布於海岸地帶的草原以及巴西東南部聖保羅郊外的Piedade山區。許多研究已經證實巴西蘑菇對於人體免疫系統具有許多益處，除了有預防疾病、增強免疫、抗氧化外，更有防癌抗腫瘤及調節免疫等功效。近年來巴西蘑菇備受矚目，主要因為其富含許多天然的生物活性物質，例如多醣體、beta-D-glucan 以及ergosterol等，其中又以多醣體相關文獻最多，因為多醣體具備抗腫瘤能力。在此研究中，主要將巴西蘑菇之常溫菌株以及高溫菌株分別在不同培養溫度下進行試驗，培養溫度範圍為20℃-40℃，每5℃為區間共5個培養溫度，並分別探討其菌體生長、多醣體生成以及β-(1,3)-D glucan含量，並比較兩株巴西蘑菇菌株間的差異，綜合以上實驗結果，找到最適菌體生長以及最適β-(1,3)-D glucan生成之溫度，再利用兩階段操作(two-stage operation)方式來增加常溫菇以及高溫菇菌體生長和提高多醣體內所含之β-(1,3)-D glucan含量。 ;In oriental culture, mushrooms has been used as health foods and home remedies for thousands of years. In several edible mushrooms, Agaricus blazei Murill was considered one of the most important edible and medicinal mushrooms native from Brazei. We also called it Agaricus brasiliensis. Many studies had proved that the Agaricus blazei Murill have strong immunomodulatory and health care functions, such as immune-enhancement, oxidation resistance, lower blood lipids et al. Recently, Agaricus blazei Murill is fascinating natural source for obtaining bioactive materials such as polysaccharides, beta-D-glucan and ergosterol. Among these chemical components, its polysaccharides with antitumor activity have attracted most attention. Therefore, the yields and functionality of bioactive properties produced by liquid fermentation are highly depend on the culture conditions and environment parameters, such as pH, medium compositions, culture temperature and aeration rate et al. Culture temperature is the one of the most important parameters influencing the production of bioactive properties and the cell growth. So, in my research I choose two different fruiting time of Agaricus blazei Murill to study on the effect of culture temperature ranging from 20℃ to 40℃ on cell growth, production of bioactive polysaccharides and beta-D-glucan. Agaricus blazei Murill were evaluated via five culture temperatures in step of 5℃ in a liquid fermentation, in order to optimize liquid fermentation condition of Agaricus blazei Murill for highest amount of biomass, bioactive polysaccharides and beta-D-glucan production and to compare what different between two strains of Agaricus blazei Murill. According to the results, use the two-stage temperature to increase the production of beta-D-glucan content.
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