摘要 皮膚是人類最大的器官，包括衰老過程中所造成的皺紋在內的幾種皮膚狀 況可能與膠原蛋白的降解有關。纖維細胞是產生膠原蛋白的主要細胞。第 1 型膠原蛋白（Col1）是人體內最豐富的膠原蛋白，為了創造一種可以刺 激皮膚中膠原蛋白生成的方式，將水稻與生活在土壤和人類皮膚中的解澱 粉芽孢桿菌一起培養。 我們的研究結果表明，水稻與解澱粉芽孢桿菌細菌 的孵育上調了γ-氨基丁酸（GABA）的水平。 局部應用 GABA 或富含 GABA 的水稻顯著增加了小鼠皮膚中 Col1 的產生。 此外，通過小干擾核 糖核酸（siRNA）體內敲除 GABA 受體亞單位β-3（GABRB3）顯著降低了 小鼠皮膚中 Col1 的產生，表明 GABRB3 介導 GABA 誘導的皮膚中 Col1 的 產生。;Abstract Skin is the largest organ in humans. Several skin conditions including wrinkles during aging could be associated with degradation of the collagen. Fibroblast is the main cell that produces collagen. Collagen type 1 (Col1) is the most abundant collagen in the human body. With an aim to create a modality that can stimulate collagen production in skin, rice was incubated with a Bacillus amyloliquefaciens (B. amyloliquefaciens) bacteria which live in both soil and human skin. Our results demonstrate that incubation of rice with B. amyloliquefaciens bacteria up-regulated the level of Gamma-aminobutyric acid (GABA). Topical application of GABA or GABA-rich rice significantly increased the production of Col1 in mouse skin. Furthermore, in vivo knockout of GABA receptor subunit beta-3 (GABRB3) by small interfering ribonucleic (siRNA) markedly reduced Col1 production in the mouse skin, indicating that GABRB3 mediates the GABA-induced Col1 production in skin.
