脂質體因具有良好的生物相容性與藥物包覆能力，長期被視為理想的藥物載體。然而，其結構穩定性不足與釋藥行為難以精準控制，限制了臨床應用。為改善此問題，本研究引入聚乙二醇二甲基丙烯酸酯（PEG-DMA）於囊泡內部光聚合形成凝膠核心，建立「凝膠核心脂質體（gel-in-liposome）」系統，並系統性探討其對脂質雙層物理性質的影響。研究方法結合動態光散射（DLS）、小角度 X 光散射（SAXS）與螢光淬滅實驗，以分析不同 PEG-DMA 含量下囊泡的結構、力學性質與曲率變化。實驗結果顯示，凝膠核心的引入不僅能維持囊泡在低比例時的穩定分散性，亦會在高比例時造成粒徑與多分散性上升，並影響膜厚、剛性及自發曲率的趨勢。整體而言，本研究提供了一套能藉由內核凝膠化來調控脂質體性質的新方法，對藥物輸送與奈米醫學設計具有參考價值。;Liposomes are widely recognized as promising drug carriers due to their biocompatibility and drug encapsulation ability. However, their limited structural stability and poorly controlled release behavior restrict broader clinical applications. To address these issues, this study incorporated poly(ethylene glycol) dimethacrylate (PEG-DMA) into vesicle interiors, where photopolymerization generated hydrogel cores to form “gel-in-liposome” systems. We systematically examined how these hydrogel networks influence bilayer properties using dynamic light scattering (DLS), small-angle X-ray scattering (SAXS), and fluorescence quenching experiments. The results showed that while vesicles maintained stable dispersion at low PEG-DMA ratios, higher ratios led to increased size and polydispersity, along with significant changes in membrane thickness, rigidity, and spontaneous curvature. Overall, this work presents an approach to modulate liposomal properties through core gelation, offering insights for the design of advanced drug delivery and nanomedicine systems.
