| dc.description.abstract | Thermoresponsive surface prepared using thermoresponsive polymers with low critical solution temperatures (LCSTs) is attractive candidates for cell culturing because cells can be detached from the surface without applying an enzymatic digestion method and, instead, by decreasing the temperature, e.g., to 4 °C, which enables cell aggregates or cell sheets to be obtained. In this study, the thermoresponsive nanobrush surfaces are designed for human stem cell culture (human adipose-derived stem cells [hADSCs] and human embryonic stem cells [hESCs]). Using RAFT polymerization, I prepared the coating copolymers having polystyrene anchor and (a) thermoresponsive poly(N-isopropyl acrylamide), PNIPAAm, (b) biocompatible polyethylene glycol methacrylate (PEGMA), and (c) polyacrylic acid (PAA) where bioactive oligopeptide (oligo-vitronectin) can be conjugated via carboxylic acid of PAA. The coating copolymers had narrow molecular weight distribution (PDI is less than 1.8) and were characterized by NMR and FTIR. The coating surface density was analyzed by XPS and SPR measurements. hADSCs were cultured on the surface coated with copolymers containing PNIPAAm, PEGMA and PAA conjugated with oligo-vitronectin. I investigated the optimization of hADSCs attachment and detachment with high efficiency in cooling process. While less than 20% of hESCs were able to detach from the commercially available control dishes, TCPS, hESCs can be cultured and detached from thermoresponsive nanobrush surfaces and maintained the pluripotency. Furthermore, hPSCs culture on thermorespsonsive nanobrush surfaces can be applied to 3D culture system for clinical application by introducing the microcarriers, which can be coated with copolymers developed in this study. | en_US |