| dc.description.abstract | Metal-organic frameworks (MOFs) are a class of porous crystalline materials composed of two main components: (i) clusters (or better multinuclear complexes) and (i) linker systems. In the past decade, they have been extensively studied because of their extraordinary porosity that makes them suitable for many applications. In particular, due to the variety of the choosing metallic clusters and organic linkers with different functional groups, MOFs have significantly physical and chemical properties. In these characters, a pretty interesting study reported by our lab about the combination of enzyme (Catalase) and MOF (ZIF-90) under a de novo approach. The ZIF-90 support provides an interesting size-sheltering function to catalase and protects catalase from the protein killer-proteinase-K. That study offers a novel tool to immobilize and impart new functions to biomolecules. In order to expand the routes for other enzymatic reactions, we choose UiO-66 (nominal composition: Zr6O4(OH)4(BDC)6; BDC = 1,4-benzene dicarboxylate) as our main material because UiO-66 with bigger aperture size at 6 Å comparing it in ZIF-90 at 3.5 Å. This property allows the bigger substrate to deliver into MOF structure for contacting embedded enzymes. Under harsh condition of the conventional way for the UiO-66 synthesis, normally, the catalytic activity of the biological component significantly is able to be deteriorated because of the chemically/thermally induced denaturation of protein. Therefore, a way of the milder condition to synthesize UiO-66 needs to be developed. In this regard, we provided a new approach, i.e., mechanochemistry, to synthesize MOF material in this study: a UiO-66 analog was synthesized in 180 s using water-assisted grinding. The linker solubility suggested that tetrafluorobenzene- 1,4-dicarboxylic acid was the best linker due to the lowest average pKa value. Zr-based Metal–organic framework nanocrystals displayed good topologies and hydrophobicities, and high water/thermal stabilities. The less amorphous complex led to higher porosities and pore volumes with a 60 min grinding time. Importantly, this mild approach for obtaining MOFs without hazardous solvents provides an additional avenue for converting biomolecules or metal nanoparticles into MOFs as composites for applications. | en_US |