| DC 欄位 |
值 |
語言 |
| DC.contributor | 生命科學系 | zh_TW |
| DC.creator | 賽提威 | zh_TW |
| DC.creator | Aditya Aryandi Setiawibawa | en_US |
| dc.date.accessioned | 2021-7-20T07:39:07Z | |
| dc.date.available | 2021-7-20T07:39:07Z | |
| dc.date.issued | 2021 | |
| dc.identifier.uri | http://ir.lib.ncu.edu.tw:444/thesis/view_etd.asp?URN=107821602 | |
| dc.contributor.department | 生命科學系 | zh_TW |
| DC.description | 國立中央大學 | zh_TW |
| DC.description | National Central University | en_US |
| dc.description.abstract | 核糖核酸酶P (RNase P)屬於核酸內切酶/核糖核蛋白家族，參與轉運核糖核酸(tRNA)的成熟作用。tRNA前驅物的成熟涉及 5′ 前導序列的切割、3′ 尾端序列的切割、內含子剪接、3′ 末端添加CCA、和轉錄後修飾。 RNase P 是負責在tRNA 前驅物的 +1 位置進行特定切割來去除 5′ 前導序列的關鍵酶。RNase P通常是由一個 RNA和一到多個蛋白質共同組成。然而，最近的研究發現了一種獨特形式的 RNase P，稱為純蛋白質 RNase P (protein-only RNase P, PRORP)，它在真核生物中非常普遍。這引起了我們對不含催化性 RNA 成分的 RNase P 如何有效切割tRNA 前驅物的興趣。研究 PRORP 切割tRNA前驅物，特別是 tRNAHis 前驅物的 5′ 前導序列的機制，我們建構了酵母菌細胞核和酵母菌線粒體 RNase P 剔除菌株，用於細胞內功能測定，並純化異源 PRORP 用於體外切割測定，尤其是它們在 tRNAHis 中的切割位點（+1 或 -1 位置）。我們的研究結果顯示: Trypanosoma brucei PRORP (TbPRORP)、Aquifex aeolicus PRORP (AqPRORP) 和Chlamydomonas reinhardtii PRORP (CrPRORP) 可以互補酵母菌細胞核 RNase P剔除菌株，但不能互補酵母菌線粒體 RNase P 剔除菌株。此外，這些 PRORP 都不能切割 tRNAsHis在 -1 位置。更多深入研究持續進行中以全面了解PRORP的作用機制。 | zh_TW |
| dc.description.abstract | RNase P belongs to a universal endonuclease/ribonucleoprotein family which is involved in precursor tRNA maturation. Maturation of a precursor tRNA involves cleavage of the 5’ leader sequence, cleavage of the 3’ trailer sequence, intron splicing, CCA addition to the 3’ end, and post-transcriptional modifications. RNase P is the key enzyme responsible for removing the 5’ leader sequence by making a specific cut at the +1 position in the precursor tRNA. Normally, RNase P consists of an RNA subunit and one to several protein subunits. However, recent studies identified a unique form of RNase P, called protein-only RNase P (PRORP), that is highly ubiquitous in eukaryotic organisms. This attracts our attention as to how an RNase P without the catalytic RNA component can efficiently cleave and process precursor tRNA. To investigate the mechanism by which PRORP cleaves the 5’ leader sequence of a precursor tRNA, in particular tRNAHis, we constructed a yeast nuclear and a yeast mitochondrial RNase P knockout (KO) strain for in vivo functional assay, and purified the heterologous PRORPs for in vitro cleavage assay, with a particular emphasis on their cleavage site in tRNAHis (+1 or -1 position). We showed herein that Trypanosoma brucei PRORP (TbPRORP), Aquifex aeolicus PRORP (AqPRORP), and Chlamydomonas reinhardtii PRORP (CrPRORP) can rescue the yeast nuclear RNase P KO strain, but none of these PRORPs can rescue the yeast mitochondrial RNase P KO strain. Moreover, none of these PRORPs can cut tRNAsHis at -1 position. A more detailed analysis is underway to characterize these PRORPs. | en_US |
| DC.subject | 核糖核酸酶P | zh_TW |
| DC.subject | 轉錄後修飾 | zh_TW |
| DC.subject | 蛋白質生物合成 | zh_TW |
| DC.subject | 轉譯 | zh_TW |
| DC.subject | 轉運核糖核酸 | zh_TW |
| DC.subject | RNase P | en_US |
| DC.subject | posttranscriptional modification | en_US |
| DC.subject | protein synthesis | en_US |
| DC.subject | translation | en_US |
| DC.subject | tRNA | en_US |
| DC.title | Functional replacement of yeast nuclear and mitochondrial RNase P by a protein-only RNase P | en_US |
| dc.language.iso | en_US | en_US |
| DC.type | 博碩士論文 | zh_TW |
| DC.type | thesis | en_US |
| DC.publisher | National Central University | en_US |