| dc.description.abstract | The wound region of second degree burn involves epidermis and portions of upper dermis and lower dermis. It is formed with blistered and swollen, and is easily infected with microbiomes, result in extremely painful. The clinical syndromes include fever, erythema, edema, suppuration. That require immediate medical treatment to avoid further complications. In this study, we aim to provide an effective strategy for an improved burn wound healing efficiency through design and fabrication of a synthetic chitosan-based hydrogel containing silver ions and epidermal growth factor (EGF) nanoparticles named as (SCPEHG) chitosan nanoparticle EGF hydrogel that may provide both antibacterial and would healing-enhanced functionalities. The EGF encapsulated chitosan nanoparticles were fabricated by using polyanionic as the coacervation crosslink agent. Through the DLS analysis, the size and surface charge of the EGF-loaded nanoparticles are 352 ± 16 nm and 20.5 ± 2.3 mV, respectively. The encapsulation efficiency and the loading rate of the EGF in the nanoparticles were 93.13% and 0.044%. The optimal effective dosages of 24 mM Ag+ and 60 ug/mL EGF for the SCPEHG manufacture were first determined based on the results of antibacterial, cytotoxicity, and cell growth examinations. When the silver ions released in 48 hours, the SCPEHG release were 6.70 mg/L and we can find over 96% cells survival in vitro experiments. The EGF hydrogel release were 1.3 ng/mL in PBS in 48 hours, we also can notice that the cell grow up more fast than in water. We characterized the optimized SCPEHG and found that the composite hydrogel was able to provide sustained release of Ag+ and EGF, and exhibited a significantly higher hydration capacities, including the swelling degree and equilibrium water content, in PBS than those in deionized water, showing that the developed SCPEHG is highly applicable in the ion-rich environment such as burns wound site. In summary, we develop the hydrogel is highly advantageous for curing burns wound and enhancing cell growth. | en_US |