| dc.description.abstract | In this research carries out the synthesis and analysis of a series of ionic liquids and applies them to the extraction and detection of biological liposoluble protein. The extraction, purification, and concentration of liposoluble protein are very important engineering issues in biomedical research and applications. The methods of extracting and separating liposoluble protein in traditional biochemical engineering include reverse microcell extraction and aqueous two-phase solvent engineering. However, those methods are still for improvement in its procedures and efficiency. Ionic liquids have special properties such as low melting point, high thermal stability, high polarity, resistance to strong acids and low vapor pressure. Ionic liquids can change the combination and change of anions and cations according to the required physical or chemical characteristics to dissolve insoluble molecules. Also, ionic liquids are safe and recyclable, which can effectively reduce environmental pollution and have the opportunity to replace the current commonly used organic solvents. Therefore, in this study, ionic liquids were used for liposoluble protein extraction and purification. Then the results were used to application and analysis.
In the synthesis and verification section, this research first synthesized and analyzed a series of ionic liquids with different structures, and explored the solubility of ionic liquids with different concentrations and structures to liposoluble protein. The results show that [1-MIM-PEG550][OMs-] has the best solubility for fat-soluble gluten protein. At room temperature, 1 ml of 40% [1-MIM-PEG550][OMs-] ionic liquid can dissolve 855ppm fat-soluble gluten protein. Also, in the verification part, the synthetic ionic liquid is used to take the fat-soluble gluten protein as an example, combined with the immune micromagnetic beads and the electrochemical system for rapid extraction and detection of liposoluble protein. In the overall detection, it only takes a few minutes to finish the extraction and detection of liposoluble protein. The correlation coefficient is 0.99 and the limit of detection is 12.6 pg/mL. Compared with the general enzyme-linked immunosorbent assay (ELISA) it greatly reduces the time of sample preparation and effectively improves the detection speed.
This research completed the synthesis and analysis of a series of ionic liquids and took the fat-soluble gluten protein as an example to successfully apply it to the extraction and detection of biological liposoluble protein. This research not only provide the technique of liposoluble protein liquid phase extraction in the future choices, but also expanded the application level of ionic liquids. | en_US |