| dc.description.abstract | Chloroethenes (CE) pollution poses a long-term and challenging issue for soil and groundwater in the world, especially since the carcinogenic compound vinyl chloride (VC) is resistant to microbial degradation in the natural environment, leading to prolonged accumulation of VC in groundwater, thus complicating the remediation efforts. This study successfully isolated a novel strain CWV2 of Dehalococcoides mccartyi (Dhc), and its consortia capable of completely dechlorinating VC. It was confirmed that this strain produces little or no vinyl chloride during the degradation of tetrachloroethylene and trichloroethylene to ethene. Using multi-omics technologies (including genomics, transcriptomics, translatomics, and proteomics), we conducted an in-depth analysis of the key reductive dehalogenase enzyme, VcrA, elucidating the molecular regulatory mechanisms involved in CEs metabolism. This offers significant strategic guidance for future efforts to enhance CEs pollution remediation in Taiwan. In the second part of the study, we applied the research at a CE-contaminated site, developing an innovative dechlorination colloidal gel combined with a permeable reactive bio-barrier (PRBB) using Dhc. Field applications confirmed that this technology achieves lasting dechlorination effects within 300 days, completely transforming CEs into non-toxic ethene, thereby demonstrating the effectiveness of this PRBB remediation method in pollution interception. In the third part, addressing the need for real-time monitoring of microbial communities in groundwater during bioremediation, we developed an environmental biochip, Dehalochip, for the rapid detection of shifts in dechlorinating bacterial communities and the effectiveness of remediation. This chip is capable of sensitive and specific detection of common dechlorination genes associated with CEs, chloroalkanes, chloromethanes, and chlorobenzenes. Through analysis of field water samples, we verified its effectiveness in real-world applications, confirming that Dehalochip serves as a reliable tool for in-situ environmental monitoring. | en_US |