| dc.description.abstract | Oral cancer is one of the top ten cancers worldwide. The global incidence of oral cancer is increasing and tends to occur at a younger age, placing a heavy burden on global medical health. Of the high incidence and mortality of oral cancer, 90% is caused by oral squamous cell carcinoma and 76% of oral cancer patients survive within 5 years without metastasis, but the incidence rate continues to rise. There are many treatment options for oral cancer,including surgery, radiotherapy and chemotherapy, but the side effects are quite large, especially surgical treatment is a non-reversible treatment, so this thesis mainly discusses the use of natural Chinese herbal medicine to develop the treatment of oral cancer.
The occurrence of oral cancer is caused by related specific gene mutations. We are trying to use the influence of Chinese herbal medicines on the genes of oral cancer cells to explore the feasibility of developing Chinese herbal medicines into oral cancer drugs. In this thesis, 27 chemotherapeutic drugs and 63 natural compounds are investigated. Most of the pharmacological effects are mainly to inhibit the growth of oral cancer cells by inducing the apoptosis pathway of oral cancer, and found that the flavonoids of natural Chinese herbal compounds can be used as anti-oral cancer drugs, so this paper will explore Daphne genkwa extract therapeutic mechanism of HGK in specific gene mutations in oral cancer cells.
Hydroxygenkwanin (HGK) is a natural flavonoid extracted from Daphne. Previous studies have shown that HGK has anti-inflammatory and anticancer effects, but its role in oral cancer is still unclear. This paper discusses that HGK flavonoids, as natural Chinese herbal medicines, can indeed affect the gene expression of cancer cells. Experiments showed that HGK dosage inhibited the growth of SAS and OCEM1 cells. Functional enrichment analysis of genes revealed important pathways for cell motility, cell cycle, and cell growth and proliferation. We demonstrated by flow cytometry that HGK induces cell cycle arrest and by bioassay that HGK inhibits cell colony formation and cell motility. Western blot showed that HGK induces cell cycle arrest and induces the intrinsic apoptotic pathway through p21 activation. HGK inhibited cell invasion and migration by downregulating Vimentin protein. HGK may be an effective natural product for the treatment of oral cancer.
In the past, many studies have investigated the effect of Chinese herbal medicine on cancer cells, but they have not looked specifically at the gene expression of cancer cells. We hope that through the effect of HGK on the gene expression of oral cancer cells, it will be possible to explore the development of natural anti-oral cancer drugs to provide a treatment for oral cancer. Risk and possibility of developing adjuvant therapy for oral cancer. | en_US |