| dc.description.abstract | The raw material of health food is mainly from animal, plant or microorganism, and this experiment chose plant source as the raw material. There are many methods of extraction such as distillation, microwave-assisted extraction, supercritical fluid extraction, etc. There are also many purification methods, such as chromatography, ion exchange, supercritical fluid technology can also be used as purification methods.
Nicotinamide mononucleotide (NMN) is a nucleotide derived from ribose and nicotinamide. NMN is made from the body′s B-complex vitamins, a naturally occurring molecule in all living organisms, and can prevent obesity, anti-aging, neuroprotection, cardiovascular disease, and other benefits.
In this study, the food with the highest content of nicotinamide mononucleotide, edamame, was used as the raw material. Extraction and purification of nicotinamide mononucleotides from natural food can be used as a new health food. Acetone and water were used as solvents to extract nicotinamide mononucleotides, and the extract was concentrated in a vacuum oven. The results show that the nicotinamide mononucleotides were more easily extracted by using water as the solvent than acetone. The size of nicotinamide mononucleotide was observed to be 50-70 nm by using transmission electron microscope. The absorption peak of nicotinamide mononucleotide was shown to be around 290 nm by UV-Vis spectroscopy. Finally, the pure nicotinamide mononucleotide was compared with edamame extract by thin layer analysis (TLC), and it was confirmed that nicotinamide mononucleotide was successfully extracted from edamame extract. | en_US |