| dc.description.abstract | There are many mechanical forces in the human body, and stretching is one of them. Cyclic stretching exists in the heart, lungs, etc. In the past, it was known that cyclic stretching would affect cell proliferation, differentiation, and gene regulation. And it plays an important role in tissue maintenance. In this study, human lung fibroblasts NHLF and lung adenocarcinoma cells A549 were treated with mechanical circulation stretching, and the degree of fibrosis, the invasion of cancer cells in a three-dimensional environment and the interaction with surrounding immune cells were explored. The results show that given a certain degree of cyclic stretching can make lung fibroblasts express more myofibroblast marker α-SMA. In the cancer cell part, cyclic stretching can inhibit the invasion of lung adenocarcinoma spheroid in the collagen hydrogel. After stretched A549 conditioned medium with a magnitude of 10% and a frequency of 0.21 hz treating, Naïve macrophages have obvious M1 markers TLR2, IL-1β, TNF-α increase and M2 marker CD206 decrease at 24 and 48 hours, which indicates that the stretched A549 may promote the macrophages to move toward M1 phenotype. | en_US |