| dc.description.abstract | Hearing loss is a common disease that can cause many inconveniences in life. There are many factors that can cause hearing loss, such as genetic defects, environmental noise, ototoxic drugs, and the effects of aging. With the ageing population problem, the number of people with hearing loss will increase from 150 million in 2019 to 250 million in 2050.Hearing loss can be divided into two types. The first is conductive hearing loss, which is usually caused by foreign body obstruction in the ear canal or fluid accumulation in the middle ear cavity. Clinically, surgical treatment or hearing aid assistance is often used to amplify sound signal. The other one is sensorineural hearing loss (SNHL), most of which is due to the damage of hair cells without regeneration, resulting in the inability of nerve signals to enter the brain At present, the common clinical treatment is to place the cochlear implant, but the cost is very high but cannot cure. Therefore, introducing cell therapy for hearing loss is a potential method. From the point of view of embryonic development, we knew the endolymphatic fluid changes from high sodium to high potassium slowly during the early stage of hair cell differentiation. The aim of the study, we use potassium alginate to add strontium chloride to cross-link to form K-beads with a semi-permeable membrane. Consequently, the K-beads can release potassium ions and reduce sodium ions to create dynamic ion regulation forming in-vitro microenvironment for hair cell differentiation. In summary, we successfully developed in-vitro dynamic ion regulation system for mimicking in-vivo endolymphatic microenvironment. | en_US |