| dc.description.abstract | Bioreactors play an integral role in bioengineering, drug development, and tissue culture. In view of the fact that many studies have pointed out that external physical stimulation can promote cell growth and differentiation, such as mechanical stretching, electrical stimulation, shear stress, etc., however, many studies are still unable to stimulate cells in a variety of external physical stimulation ways, and in many cases The physical force stimulation bioreactor designed in the study has a relatively single functionality and low variability. The overall equipment cannot be modularized, which limits the scalability of the equipment. In addition, there is no good control system support, resulting in In the eyes of the user, the equipment becomes a bioreactor dedicated to specific cells or tissues, which greatly limits the breadth of use. Most of the commercial physical force stimulation bioreactors sold on the market today are also Only a single stimulation mode is available. Only a few manufacturers of bioreactors have bioreactors that provide multiple physical force stimulations, can perform good cell culture, and have good control systems to provide a place that simulates the natural growth environment of cells. However, the biggest disadvantage of commercially available bioreactors is that they are expensive and are not suitable for use by most research institutions.
Therefore, this paper will try to use polymethylmethacrylate (PMMA) to make the device body, and use polydimethylsiloxane (PDMS) film with polypyrrole (PPy) deposited as the cell culture carrier to design a modular A composite physical force stimulation bioreactor, its stimulation mode combines electrical pulse stimulation and pneumatic stretch stimulation, and its equipment and stimulation mode are modularized to increase the versatility and expansion of the equipment, making it available to users According to the experimental needs, the Raspberry Pi is used as the control center in the control system, and the designed control interface is used to control the parameters of stretching stimulation and electrical stimulation. Finally, a low-cost modular composite device is completed. Physical forces stimulate the bioreactor. | en_US |