| dc.description.abstract | Colon carcinoma is one of the most fatal and common gastrointestinal cancers in the world. Cancer-initiation cells (CICs) or cancer stem cells (CSCs), are responsible for tumor initiation, growth, and metastasis. The purification and isolation of tumorigenic colon CSCs (CICs) from primary colon tumor tissues should be valuable for the development of novel diagnostic and personal therapeutic treatments in the future. In this study, CSCs (CICs) of colon carcinoma cell line CoLo205 and SW480 (model of primary colon carcinoma cells) were purified from human fibroblasts (CG1639). Furthermore, the primary colon carcinoma cells were purified from colon tumor tissues of patients utilizing the membrane filtration method via nylon mesh filters and poly(lactide-co-glycolic acid)/silk screen (PLGA/SK) filters. In the separation of colon carcinoma cells from fibroblasts, both cells were stained with Cell Tracker Red and Blue, respectively for their identification using flow cytometry. CoLo205 purity was enhanced from 50% to 80% in the recovery fraction, which showed the higher cancer cells remaining. The isolation efficiency was characterized using (i) CD44 and CD133 marker expression, (ii) colony-forming unit assay, and (iii) carcinoembryonic antigen (CEA) production. Especially, CSCs (CICs) were purified in the migrated cells compared to the cells in permeation solution and recovery solution, when CoLo205 or SW480 cells were permeated through the membranes. However, the migrated cells generated similar efficiency of colony forming unit compared to the cells in the permeate solution and recovery solution. The colon cancer cells remained in the recovery solution, when the mixed solution of colon cancer cells and fibroblasts were permeated through the membranes. Therefore, a high proportion of colon CSCs (CICs) from the patient’s tumor tissue is expected to be isolated using the membrane filtration method developed in this study. | en_US |