| dc.description.abstract | Osteoarthritis is the most common type of arthritis in the world. It occurs due to the wear, degeneration, and dehydration of cartilage in the joints. Anyone can get the disease, and osteoarthritis becomes more common as you get older. It makes people pay attention to their health earlier, leading to a gradual increase in the global market demand for dietary supplements.
Schizophyllum is a kind of edible and medicinal mushroom, and the exopolysaccharide produced by Schizophyllum during liquid fermentation is called schizophyllan. Glucosamine is a component of polysaccharides and an important component in the composition of human joints. Glucosamine is often used as a dietary supplement for osteoarthritis. It is believed that supplementing with glucosamine can lubricate joints, repair cartilage, and increase cartilage production.
Based on the above advantages, in order to effectively increase the production of glucosamine and shorten the production time, the strain used is Schizophyllum commune BCRC 37951. Studies have confirmed that glutamic acid can effectively provide energy to some microorganisms to facilitate the growth of the bacteria. Therefore, this study chose to add glutamic acid, and explored various fermentation conditions ― the amount of glutamic acid added, different carbon sources, initial pH value, and fermentation temperature. Optimal conditions were carried out according to the parameters of bacterial growth activity, glucosamine production and antioxidant activity.
In this study, the optimal conditions were added ― 0.3% glutamic acid, 3% fructose, initial pH value 4.5 and fermentation temperature 25°C. Under these conditions, Schizophyllum commune BCRC 37951 showed 896.22 mg/L glucosamine, 2038.87 mg GA/L TPC, and 80.5% DPPH scavenging activity. | en_US |