| dc.description.abstract | As people become more concerned about health and their health awareness increases, the
health food market continues to grow, becoming an important part of daily life. It is widely
used to boost immunity, promote metabolism, improve sleep, and delay aging. Against this
backdrop, nicotinamide mononucleotide (NMN) has attracted widespread attention as a
potential health supplement. NMN, a precursor of nicotinamide (vitamin B3), plays a crucial
role in cellular metabolism and energy production and is a key biosynthetic pathway for the
synthesis of nicotinamide adenine dinucleotide (NAD+). Research has shown that NMN
supplementation can increase NAD+ levels, thereby enhancing cellular vitality, improving
metabolic health, delaying aging, and increasing exercise endurance. Consequently, it is
considered a promising health food ingredient. This study aims to optimize the ribose
phosphorylation reaction steps to achieve efficient and economical NMN production. Initially,
ribokinase is used to react ribose with adenosine triphosphate (ATP) to generate ribose-5
phosphate and adenosine diphosphate (ADP). Subsequently, polyphosphate kinase II (PPK2)
is employed to react the by-product ADP from the ribose phosphorylation reaction with long
chain polyphosphate (polyP) to regenerate ATP. By coupling these two reactions, a simple and
efficient multi-enzyme catalytic system is established. This research optimizes the reaction
conditions such as temperature, pH, and buffer to establish a one-pot synthesis of NMN.
Additionally, in the synthesis of NMN, varying the substrate concentration, enzyme
concentration, and ionic strength increases the NMN yield. Through systematic experimental
design and data analysis, the conditions for the ribose phosphorylation reaction were
successfully optimized, achieving efficient NMN production. | en_US |