| dc.description.abstract | Cardiovascular diseases presently rank among the top three leading global causes of mortality, with heart failure representing a significant contributor to this high mortality rate. Consequently, it is imperative to develop research models that accurately simulate heart failure. Cellular behavior within the human body dynamically interacts with its microenvironment, making it essential to recreate this interaction in experimental settings. In this study, a three-dimensional scaffold constructed from porcine gelatin methacryloyl was employed to replicate the in vivo microenvironment through mechanical cyclic stretching and compression. These mechanical conditions involved a 10% strain and a 1.5 Hz frequency. The research investigated the impact of mechanical cyclic loading within the three-dimensional scaffold on AC16 human cardiomyocyte cells. The findings reveal that AC16 cells cultured within dynamic three-dimensional scaffolds exhibited heightened expression of F-actin, TGF-β, and YAP at three and seven days, providing confirmation that 3D biomimetic dynamic culture fosters cellular growth. In the dynamic three-dimensional scaffold, the AC16 cells cultured showed an increase in the expression levels of BNP, CRP, desmin, myosin, and TNNI3, which are associated with heart failure, on the 3rd and 7th days. Additionally, the impact of miRNA on the cells was observed in the exosomes derived from the cells. | en_US |