| DC 欄位 |
值 |
語言 |
| DC.contributor | 環境工程研究所 | zh_TW |
| DC.creator | 高橋拓實 | zh_TW |
| DC.creator | Takumi Takahashi | en_US |
| dc.date.accessioned | 2025-3-26T07:39:07Z | |
| dc.date.available | 2025-3-26T07:39:07Z | |
| dc.date.issued | 2025 | |
| dc.identifier.uri | http://ir.lib.ncu.edu.tw:444/thesis/view_etd.asp?URN=112326601 | |
| dc.contributor.department | 環境工程研究所 | zh_TW |
| DC.description | 國立中央大學 | zh_TW |
| DC.description | National Central University | en_US |
| dc.description.abstract | 黃素腺嘌呤二核?酸 (FAD) 是許多生物氧化還原和自由基反應中的關鍵輔助因子。傳統上，核黃素的 FAD 生物合成遵循兩步驟?途徑，需要 ATP，以黃素單核?酸 (FMN) 為中間體。工業規模的 FAD 生產傳統上依賴微生物發酵，但這些方法通常需要繁瑣的淨化步驟。考慮到原子經濟性和產量效率的重要性，酵素體外方法提供了一種更可持續和有效的選擇。然而，由於依賴昂貴的 ATP 作為底物，?促 FAD 合成的廣泛工業應用受到阻礙。為了解決這個限制，我開發了一種雙?級聯繫統，利用來自廢水微藻的聚磷酸鹽作為磷酸鹽供體，從腺?中再生 ATP。此再生機制透過雙功能核黃素激?/FAD合成?和焦磷酸?的共同作用，使核黃素在2小時內完全轉化為FAD，最終濃度達到約1.2g/L（1.5mmol/L）。值得注意的是，唯一的副產品正磷酸鹽可以透過廢水微藻回收來再生聚磷酸鹽，進而可以作為磷酸鹽供體重新融入系統。這種閉環策略有利於實現環境永續的 FAD 合成過程，同時最大限度地減少廢棄物產生。 | zh_TW |
| dc.description.abstract | Flavin adenine dinucleotide (FAD) serves as a crucial cofactor in numerous biological redox
and radical reactions. Conventionally, FAD biosynthesis from riboflavin follows a two-step
enzymatic pathway requiring ATP, with flavin mononucleotide (FMN) as the intermediate.
Industrial-scale FAD production has traditionally relied on microbial fermentation, but these
methods often entail laborious purification steps. Given the importance of atomic economy and
yield efficiency, an enzymatic in vitro approach presents a more sustainable and effective
alternative. However, the widespread industrial application of enzymatic FAD synthesis is
hindered by its dependence on costly ATP as a substrate. To address this limitation, I have
developed a two-enzyme cascade system that regenerates ATP from adenosine, utilizing
polyphosphate derived from wastewater microalgae as a phosphate donor. This regeneration
mechanism enables the complete transformation of riboflavin into FAD within 2 h, achieving
a final concentration of approximately 1.2 g/L (1.5 mmol/L) through the combined action of
bifunctional riboflavin kinase/FAD synthetase and pyrophosphatase. Notably, the only
byproduct, orthophosphate, can be recycled by wastewater microalgae to regenerate
polyphosphate, which in turn can be reintegrated into the system as a phosphate donor. This
closed-loop strategy facilitates an environmentally sustainable FAD synthesis process with
minimal waste production. | en_US |
| DC.subject | 黃素腺嘌呤二核?酸（FAD） | zh_TW |
| DC.subject | ?催化合成 | zh_TW |
| DC.subject | 永續生物催化 | zh_TW |
| DC.subject | 核黃素激? | zh_TW |
| DC.subject | PLP 依賴型消旋? | zh_TW |
| DC.subject | 綠色化學 | zh_TW |
| DC.subject | 生化工程 | zh_TW |
| DC.subject | Flavin adenine dinucleotide (FAD) | en_US |
| DC.subject | Enzymatic synthesis | en_US |
| DC.subject | Sustainable biocatalysis | en_US |
| DC.subject | Riboflavin kinase | en_US |
| DC.subject | PLP-dependent racemase | en_US |
| DC.subject | Green chemistry | en_US |
| DC.subject | Biochemical engineering | en_US |
| DC.title | 一鍋法完全酵素生成黃素腺嘌呤二核?酸的研究及第一個PLP依賴性二氨基丙酸消旋?的分子表徵 | zh_TW |
| dc.language.iso | zh-TW | zh-TW |
| DC.title | Studies on the one-pot complete enzymatic generation of flavin adenine dinucleotide and the molecular characterization of the first PLP-dependent diaminopropionic acid racemase | en_US |
| DC.type | 博碩士論文 | zh_TW |
| DC.type | thesis | en_US |
| DC.publisher | National Central University | en_US |