| dc.description.abstract | In this study, the synthesis and characterization of a novel galactosyl chitosan are reported. The novel galactosyl chitosan was prepared by covalent coupling of lactobionic acid with chitosan(gal-chit) ; and galactosyl chitosan was prepared by introducing a L-lysine spacer to chitosan,followed by covalent coupling of lactobionic acid with the lysine spacer on chitosan(gal-lys-chit) ,and witch maked into membranes and nano-particles experiment.
Morphology and mechanical properties of membranes were studied by FTIR analysis, X-ray diffraction, swelling study, antibacterial test, bind galectin-1 and cell train. Nano-particles were studied by FTIR analysis, X-ray diffraction ,nano-particles sizes , zeta potential and target to liver cells.
In the film experiment, improve the ability to resist fungus of the gal-chit and gal-lys-chit membrane materials, because the lactobionic acid is base group of the hydrophile; and the ability to effectively bind galectin-1(Gal-1); galactose and surface receptor of liver cell can lead sticking to the growing type attitude on the material of liver cell.
In the nano-particles experiment, gal-chit,gal-lys,gal-lys-chit of the value are relatively high of the nano-paticles and zeta potential ,because three dimensional obstacle of the galactose of ring structure will influence effort of the electric charge between positive electricity amine base on the chitosan and negative electricity of TPP.The nano-particles form is affected by PH value and concentration of TPP.Nano-particles at some of set time observed to target cells of liver, the longer time is gal-lys-chit NPs has more apparent result than gal-chit NPs. Can be as efficiency assessing and evidence of enduring liver cell that the rice particle marks. | en_US |