| dc.description.abstract | Filamentous fungi refers to a type of fungus, is composed of filamentous hyphae. Fungi were very important resources in terms of human, and its considerable multi-functional derivatives, such as the growth process of the metabolites produced in our health care, agriculture and industries are a very important position. At present, the fungus has been considerable academic research, which is immobilizing fungi; immobilized cell is to achieve the purpose of recycling. Immobilized cell by cell growth can save time and reduce the consumption of nutrients, and can re-use will be produced by fungal metabolites to increase the yield, but generally the use of fixed method of non-woven or cotton cloth will affect the fungi Growth of the case, the morphology changed after the cell is bound to affect the metabolism of fungi.
In this study, belonging to the filamentous fungi Schizophyllum commune for the study of the spindle, the first use of magnetic particles (Fe3O4) to recover the cell to achieve the purposes immobilized, and the use of Fed-batch 1 time the way to explore its metabolites yield of polysaccharide and with the general approach of the fixed (non-woven) to compare.
The results showed that magnetic particles (Fe3O4) can be successfully used for recovery of Schizophyllum commune, and the implementation of 1 Fed-batch experiments, we found that production of Schizophyllum commune polysaccharide than 2.4 times the high batch production rate increased about 3 times , and immobilized in general are not effective methods to improve content and product yield.
In addition, among the Fed-batch, we reduce the nitrogen source, the amount of polysaccharide can be found in the polysaccharide than the batch volume about 3 times than not reduce the nitrogen 2.4 times better, the product of unit cell of the conversion rate of Yp / x can also be upgraded to 1.7 times, and general methods of no fixed effect.
Fe3O4 reason Schizophyllum commune recovery because of its will in the cell surface adhesion, the simple use of qualitative research methods to study the adhesion behavior, and other filamentous fungi Aspergillus niger and Penicillim brevicompactum to verify.
In order to improve on Fe3O4 fungal shedding from the shortcomings of this study, the temperature sensitivity of magnetic particles (PolyNIPAAm-Fe3O4) to carry out the recovery of Schizophyllum commune, the magnetic particles have a LCST (Lower Critical Solution Temperature) in nature. The results showed that at room temperature at 25 ℃ PolyNIPAAm-Fe3O4 does not have the phenomenon of bacterial adsorption, and the ambient temperature to 50 ℃ when the act will be adsorbed.
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