| DC 欄位 |
值 |
語言 |
| DC.contributor | 生命科學系 | zh_TW |
| DC.creator | 陳念慈 | zh_TW |
| DC.creator | Nien-tzu Chen | en_US |
| dc.date.accessioned | 2010-6-25T07:39:07Z | |
| dc.date.available | 2010-6-25T07:39:07Z | |
| dc.date.issued | 2010 | |
| dc.identifier.uri | http://ir.lib.ncu.edu.tw:444/thesis/view_etd.asp?URN=972204017 | |
| dc.contributor.department | 生命科學系 | zh_TW |
| DC.description | 國立中央大學 | zh_TW |
| DC.description | National Central University | en_US |
| dc.description.abstract | 酵母菌Saccharomyces cerevisiae中cytoplasmic glutamyl-tRNA synthetase (cGluRS) 是由細胞核基因GUS1解碼而來。在細胞質中cGluRS會將Glu接到相對應的tRNAGlu形成Glu-tRNAGlu，但是最近的研究報告指出cGluRS也會被送到粒腺體中參與Gln-tRNAGln的形成。在粒腺體中cGluRS首先會將Glu接到tRNAGln形成Glu-tNRAGln，再藉由Glu-tNRAGln amidotransferase的轉胺作用形成正確的Gln-tRNAGln。先前，我們實驗室指出，在細胞質中與cGluRS結合的分子Arc1p有粒腺體標的訊號，因此cGluRS有可能是藉由Arc1p而進入粒腺體的，經由點突變的實驗我們發現cGluRS和Arc1p各自攜帶粒腺體標的訊號，不需靠彼此交互作用而進入粒腺體。cGluRS的粒腺體標的訊號位於其胺基端1-29胺基酸。相反地，在酵母菌Schizosaccharomyces pombe及Candida albicans的cGluRS中並沒有發現類似的粒腺體標的訊號。而在Pichia guilliermodii、Candida albicans、Schizosaccharomyces pombe這三株酵母菌的Arc1p也沒有粒腺體標的訊號。
| zh_TW |
| dc.description.abstract | In Saccharomyces cerevisiae, the cytoplasmic glutamyl-tRNA synthetase (cGluRS) is encoded by GUS1. In cytoplasm, cGluRS attaches Glu to the cognate tRNAGlu to form Glu-tRNAGlu. In addition to the cytoplasmic activity, cGluRS can enter the mitochodria to synthesize Glu-tNRAGln (a misacylated product), which is then converted to correct Gln-tRNAGln by Glu-tNRAGln amidotransferase. A pervious study in our lab indicated that Arc1p, which binds cGluRS in cytoplasm, also has a mitochondrial targeting signal. Our results showed that cGluRS and Arc1p each carry a mitochondrial targeting signal and thus can enter the mitochondria by itself. Next, we used the cytoplasmic form of valyl-tRNA synthetase as the reporter gene to map the mitochondrial targeting signal of cGluRS. As it turned out, this signal was embedded in the N-terminal amino acids 1-29 of cGluRS. In contrast, no mitochondrial targeting signal was found in Schizosaccharomyces pombe or Candida albicans GluRS. Moreover, Arc1p from Pichia guilliermodii, Candida albicans, and Schizosaccharomyces pombe does not possess a mitochondrial targeting signal.
| en_US |
| DC.subject | 粒腺體標的訊號 | zh_TW |
| DC.subject | GluRS | en_US |
| DC.subject | MTS | en_US |
| DC.title | 探討酵母菌細胞質 glutamyl-tRNA synthetase 的粒腺體標的訊號 | zh_TW |
| dc.language.iso | zh-TW | zh-TW |
| DC.title | Identification of the mitochondrial targeting signal of yeast glutamyl-tRNA synthetase | en_US |
| DC.type | 博碩士論文 | zh_TW |
| DC.type | thesis | en_US |
| DC.publisher | National Central University | en_US |