dc.description.abstract | Mesenchymal stem cells (MSCs) being an alternative of chondrocytes is a serious issue for cartilage repair. Even though various researches contributed to the chondrogenetic materials, there were still specifically short discussion about the surface-to-cell interaction which could brought differentiation and proliferation. However, it’s important for developing chondrogenetic material. For this study, we thought chondrocyte-to-matrix physiology might model the strategy for the usage of MSCs for chondrogenesis. For example, at articular cartilage, chondrocytes are sphere-like in vivo of the hyaline cartilage, while flat in fibrocartilage around the bone tissue as via endochondral ossification. Similarly in vitro, the dedifferentiated chondrocytes flattened by binding exactly through integrins to substrate. Moreover, the mature chondrocytes tend to aggregate in vitro. In short, the adhesion to the matrix affects physiology of chondrocytes.
Thus, we hypothesized that the adhesivity of the substrate might direct the differentiation of MSCs by transforming their adhesivity and naturally spreading morphology in 2-dimensional culture. Under this hypothesis, we modified type I collagen substrate by various amounts of non-adhesive PEG and zwitterions (iodopropionic acid, IPA) and produced surfaces of various adhesivities to affect MSCs. Through the different chemistry of surface, we would like to illustrate the interaction between cells to the matrix. The density of modification were determined by XPS, and results indicated concentration-dependent modification, following controlled the adhesion of cells. The cell morphologies were observed under optical microscope. The morphologies were distinguishingly different between the different modification that took advantages with the different surface chemistry. Most important of all, the differentiation to chondrocytes was determined by analyzing genes such as type II collagen, aggrecan, and Sox9 through RT-PCR. The results indicated that the surface adhesivity of substrate does affect the chondrogenesis of MSCs.
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