液化澱粉芽孢桿菌用於產生富含GABA的水稻以增強小鼠皮膚中膠原蛋白表達的可能機制

以作者查詢圖書館館藏

、以作者查詢臺灣博碩士

、以作者查詢全國書目

、勘誤回報

、線上人數：65

、訪客IP：3.145.201.73

姓名

颯雅(Zayabileg Altansukh) 查詢紙本館藏

畢業系所

生醫科學與工程學系

論文名稱

液化澱粉芽孢桿菌用於產生富含GABA的水稻以增強小鼠皮膚中膠原蛋白表達的可能機制
(Possible mechanisms employed by Bacillus amyloliquefaciens to generate GABA-rich rice for enhancement of collagen expression in mouse skin)

相關論文

★ Intelligent nature-derived coordinative hydrogel incorporated with HRP as dressing for infected wounds	★ 表皮葡萄球菌在人類皮膚微生物總體對皮膚訊號與腦波訊號影響
★ 土壤微生物組體研究：藉由內生細菌誘導之高GABA含量水稻增加神經肽Y以及減輕小鼠焦慮	★ Fermentation of Leuconostoc mesenteroides reduces abdominal fat accumulation in high-fat diet mice
★ 選擇性發酵引發劑（SFI）觸發表皮葡萄球菌發酵以緩解UV-B誘導的自由基生成	★ Identify and characterize the fermenting and electrogenic skin bacteria using selective prebiotics
★ 有益微生物的真菌學和細菌學研究：在農業和人類健康中的應用	★ 人體皮膚致電微生物組通過調節鐵和自由基來減輕紫外線B引起的皮膚損傷。
★ 微生物組中的細菌作為治療人類疾病的生物療法	★ 皮膚表皮葡萄球菌作為電力活性菌以抑制痤瘡丙酸桿菌
★ 鼠李糖乳桿菌作為益生菌對抗 SARS-CoV-2 膜糖蛋白誘導的炎症	★ Flavin mononucleotide-based electricity production by Staphylococcus epidermidis alleviates SARS-CoV-2- Nucleocapsid Phosphoprotein-induced IL-6 expression
★ Profiling the Age-related Microbiome via Detection of Antibodies to Gut Bacteria	★ 基於PEG的益生元影響皮膚細菌和皮膚電的發酵
★ 基於PEG的益生元對痤瘡痤瘡桿菌的表皮葡萄球菌發酵和電的研究	★ 設計開發全氟碳複合奈米藥物載體對體表微生物多效抑菌功能之研究

檔案

[Endnote RIS 格式]

[Bibtex 格式]

[相關文章]

[文章引用]

[完整記錄]

[館藏目錄]

[檢視]

[下載]

本電子論文使用權限為同意立即開放。
已達開放權限電子全文僅授權使用者為學術研究之目的，進行個人非營利性質之檢索、閱讀、列印。
請遵守中華民國著作權法之相關規定，切勿任意重製、散佈、改作、轉貼、播送，以免觸法。

摘要(中)

摘要
皮膚是人類最大的器官，包括衰老過程中所造成的皺紋在內的幾種皮膚狀
況可能與膠原蛋白的降解有關。纖維細胞是產生膠原蛋白的主要細胞。第
1 型膠原蛋白（Col1）是人體內最豐富的膠原蛋白，為了創造一種可以刺
激皮膚中膠原蛋白生成的方式，將水稻與生活在土壤和人類皮膚中的解澱
粉芽孢桿菌一起培養。我們的研究結果表明，水稻與解澱粉芽孢桿菌細菌
的孵育上調了γ-氨基丁酸（GABA）的水平。局部應用 GABA 或富含
GABA 的水稻顯著增加了小鼠皮膚中 Col1 的產生。此外，通過小干擾核
糖核酸（siRNA）體內敲除 GABA 受體亞單位β-3（GABRB3）顯著降低了
小鼠皮膚中 Col1 的產生，表明 GABRB3 介導 GABA 誘導的皮膚中 Col1 的
產生。

摘要(英)

Abstract
Skin is the largest organ in humans. Several skin conditions including wrinkles
during aging could be associated with degradation of the collagen. Fibroblast is
the main cell that produces collagen. Collagen type 1 (Col1) is the most
abundant collagen in the human body. With an aim to create a modality that can
stimulate collagen production in skin, rice was incubated with a Bacillus
amyloliquefaciens (B. amyloliquefaciens) bacteria which live in both soil and
human skin. Our results demonstrate that incubation of rice with B.
amyloliquefaciens bacteria up-regulated the level of Gamma-aminobutyric acid
(GABA). Topical application of GABA or GABA-rich rice significantly
increased the production of Col1 in mouse skin. Furthermore, in vivo knockout
of GABA receptor subunit beta-3 (GABRB3) by small interfering ribonucleic
(siRNA) markedly reduced Col1 production in the mouse skin, indicating that
GABRB3 mediates the GABA-induced Col1 production in skin.

關鍵字(中)

★ 白飯
★ 液化澱粉芽孢桿菌
★ γ-氨基丁酸
★ 第一型膠原蛋白

關鍵字(英)

★ Rice
★ Bacillus amyloliquefaciens (B. amyloliquefaciens)
★ Gammaaminobutyric acid (GABA)
★ Collagen type 1

論文目次

Contents
Chinese abstract...................................................................................................i
English abstract...................................................................................................ii
Acknowledge.......................................................................................................iii
Contents...............................................................................................................iv
List of figures.......................................................................................................v
List of tables........................................................................................................vi
List of abbreviations..........................................................................................vii
Chapter 1: Introduction......................................................................................1
1.1 Skin...................................................................................................1
1.2 Wrinkle.............................................................................................2
1.3 Collagen............................................................................................4
1.4 Gamma-Aminobutyric acid..............................................................6
1.5 Nutrition of rice................................................................................7
1.6 Bacillus amyloliquefaciens...............................................................9
Chapter 2: Materials and methods..................................................................11
2.1 Materials.........................................................................................11
2.2 Inoculation and Bacterial culture....................................................13
2.3 Rice plant preparation.....................................................................13
2.4 Extraction and purification of IAA.................................................13
2.5 Quantification of GABA content in rice grain................................14
2.6 Animal experiment..........................................................................14
2.6.1 Chemical administrations and behavioral analysis...............14
2.7 Protein extraction and measurement...............................................15
2.7.1 Preparation of lysate from tissues.........................................15
2.7.2 Determination of protein concentration................................15
2.8 Immuniblotting analysis..................................................................15
2.8.1 Polyacrylamide gel electrophoresis......................................15
2.8.2 Western blotting...................................................................16
2.8.3 Immunostaining....................................................................16
2.8.4 Densitometry analyze...........................................................17
2.9 Statistical analysis...........................................................................17
Chapter 3: Results.............................................................................................18
3.1 Enhancement of collagen formation in the mouse skin by
commercial GABA.........................................................................18
3.2 GABA concentration of rice..........................................................19
3.3 GABA concentration of rice in calcium phosphate agar plates...20
3.4 Quantitative Analysis of Indole-3-acetic acid...............................21
3.5 Enhancement of collagen formation in the mouse skin by GABArich rice...........................................................................................22
3.6 Enhancement of the collagen formation in mouse skin by GABArich rice (using CaP agar plates).....................................................23
3.7 The expression of collagen 1 in the GABRB3 receptor blocked by
siRNA in mouse skin......................................................................24
Chapter 4: Discussion and Conclusion............................................................25
References..........................................................................................................27

參考文獻

References
1. Prost-Squarcioni C1, Fraitag S, Heller M, Boehm N. Functional histology of dermis
2008 Jan;135(1 Pt 2):1S5-20. doi: 10.1016/S0151-9638(08)70206-0
2. Mosby′s Medical, Nursing & Allied Health Dictionary (4th ed.). St. Louis: Mosby.
1994. pp. 998, 774, 1497. ISBN 978-0801672255.
3. Principles of Anatomy and Physiology, Gerard J Tortora, John Wiley & Sons Inc.
Chapter 5– The Integumentary System (pg 139 – 145)
4. James, William; Berger, Timothy; Elston, Dirk (2005) Andrews′ Diseases of the Skin:
Clinical Dermatology (10th ed.). Saunders. pp. 2–3. ISBN 0-7216-2921-0.
5. McGrath, J.A.; Eady, R.A.; Pope, F.M. (2004). Rook’s Textbook of Dermatology (7th
ed.). Blackwell Publishing. pp. 3.1–3.6
6. Wilkinson, P.F. Millington, R. (2009). Skin (Digitally printed version ed.).
Cambridge: Cambridge University Press. pp. 49–50. ISBN 978-0-521-10681-8.
7. Elias PM, Goerke J, Friend DS. Mammalian epidermal barrier layer lipids:
composition and influence on structure. J Invest Dermatol 1977;69(6):535—46.
8. McCracken, Thomas (2000). New Atlas of Human Anatomy. China: Metro Books. pp.
1–240. ISBN 978-1-58663-097-3.
9. Liu X, Zhao Y, Gao J, et al. (February 2004). "Elastic fiber homeostasis requires lysyl
oxidase-like 1 protein". Nat. Genet. 36 (2): 178–82. doi:10.1038/ng1297. PMID
14745449
10. Quan, T.; Fisher, G.J. Role of age-associated alterations of the dermal extracellular
matrix microenvironment in human skin aging: A mini-review. Gerontology 2015, 61,
427–434.
11. Fisher, G.J.;Wang, Z.Q.; Datta, S.C.; Varani, J.; Kang, S.; Voorhees, J.J.
Pathophysiology of premature skin aging induced by ultraviolet light. N. Engl. J. Med.
1997, 337, 1419–1428.
12. Fisher, G.J.; Varani, J.; Voorhees, J.J. Looking older: Fibroblast collapse and
therapeutic implications. Arch. Dermatol. 2008, 144, 666–672. [CrossRef] [PubMed]
13. Fisher, G.J.; Quan, T.; Purohit, T.; Shao, Y.; Cho, M.K.; He, T.; Varani, J.; Kang, S.;
Voorhees, J.J. Collagen fragmentation promotes oxidative stress and elevates matrix
metalloproteinase-1 in fibroblasts in aged human skin. Am. J. Pathol. 2009, 174, 101–
114.
14. Varani, J.; Schuger, L.; Dame, M.K.; Leonard, C.; Fligiel, S.E.; Kang, S.; Fisher, G.J.;
Voorhees, J.J. Reduced fibroblast interaction with intact collagen as a mechanism for
depressed collagen synthesis in photodamaged skin. J. Investig. Dermatol. 2004, 122,
1471–1479.
15. Rui Yin, Qiquan Chen and Michael R Hamblin. Skin aging and photoaging. Pages 1-1
to 1-4
16. Pavida Pittayapruek , Jitlada Meephansan, Ornicha Prapapan, Mayumi Komine, and
Mamitaro Ohtsuki Role of Matrix Metalloproteinases in Photoaging and
Photocarcinogenesis
17. Nowsheen Goonoo, Dhanjay Jhurry, In vitro and in vivo cytocompatibility of
electrospun nanofiber scaffolds for tissue engineering applications. 2014.07
18. Sylvie Ricard. Blum, The Collagen family, Cold spring harbor perspective in biology,
2011.01 (3)
19. Mitsuo Yamauchi1 and Marnisa Sricholpech, Lysine post-translational modifications
of collagen, Essays Biochem. 2012 ; 52: 113–133. doi:10.1042/bse0520113
20. Lodish H, Berk A, Zipursky SL, et al. New York: W. H. Freeman; 2000. Section 22.3
Collagen: The Fibrous Proteins of the Matrix, Molecular Cell Biology. 4th edition
21. Marlyn Wu; Jonathan S. Crane Sampson Regional Med Ctr / Campbell Univ
Biochemistry, Collagen Synthesis NBK507709PMID: 29939531
22. Henning Langberg, Dorthe Skovgaard, Lars J Petersen, Jens Bülow, and Michael
Kjær, Type I collagen synthesis and degradation in peritendinous tissue after exercise
determined by microdialysis in humans, J Physiol. 1999 Nov 15; 521(Pt 1): 299–306.
doi: 10.1111/j.1469-7793.1999.00299.x
23. Roth RJ, Cooper JR, Bloom FE, The Biochemical Basis of
Neuropharmacology.Oxford University Press. 2013 :p.106.
24. Anne-Marie Sapse. Molecular Orbital Calculations for Amino Acids and Peptides.
BirkhÃ¤user, 2000. ISBN 0817638938.
25. Devashis Majumdar and Sephali Guha. Conformation, electrostatic potential and
pharmacophoric pattern of GABA (gamma-aminobutyric acid) and several GABA
inhibitors. Journal of Molecular Structure: THEOCHEM 1988, 180, 125-140.
doi:10.1016/0166-1280(88)80084-8
26. Roth, Robert J.; Cooper, Jack R.; Bloom, Floyd E. (2003). The Biochemical Basis of
Neuropharmacology. Oxford [Oxfordshire]: Oxford University Press, 416 pages.
ISBN 0-19-514008-7.
27. Alan L Pehrson, Connie Sanchez, Altered γ-aminobutyric acid neurotransmission in
major depressive disorder: a critical review of the supporting evidence and the
influence of serotonergic antidepressants
28. Richard W Olsen and Timothy M DeLorey. GABA Synthesis, Uptake and Release,
Basic Neurochemistry: Molecular, Cellular and Medical Aspects. 6th edition.
29. Möykkynen T, Uusi-Oukari M, Heikkilä J, Lovinger DM, Lüddens H, Korpi ER.
Magnesium potentiation of the function of native and recombinant GABAA receptors.
Neuroreport. 2001 Jul 20;12(10):2175-9. PMID: 11447329
30. Connolly CN, Krishek BJ, McDonald BJ, Smart TG, Moss SJ (January 1996).
"Assembly and cell surface expression of heteromeric and homomeric gammaaminobutyric acid type A receptors". The Journal of Biological Chemistry. 271 (1):
89–96. doi:10.1074/jbc.271.1.89. PMID 8550630.
31. Rang, H. P.; Dale, M. Maureen; Ritter, James M.; Flower, Rod J.; Henderson, Graeme
(2016). Rang and Dale′s Pharmacology (8th ed.). Elsevier, Churchill Livingstone. p.
462. ISBN 978-0-7020-5362-7. OCLC 903234097
32. Cossart R, Bernard C, Ben-Ari Y (February 2005). "Multiple facets of GABAergic
neurons and synapses: multiple fates of GABA signalling in epilepsies". Trends in
Neurosciences. 28 (2): 108–15. doi:10.1016/j.tins.2004.11.011. PMID 15667934
33. Han D1, Kim HY, Lee HJ, Shim I, Hahm DH, Wound healing activity of gammaaminobutyric Acid (GABA) in rats, J Microbiol Biotechnol. 2007 Oct;17(10):1661-9
34. Eriko Uehara, Hideki Hokazono, Takako Sasaki, Hidekatsu Yoshioka Faculty of
Medicine, Department of Matrix Medicine, Oita University, Yufu, Japan & Noritaka
Matsuo, Effects of GABA on the expression of type I collagen gene in normal human
dermal fibroblasts
35. Scutt A, Meghji S, Harvey W. Stimulation of human fibroblast collagen synthesis in
vitro by gamma-aminobutyric acid
36. Uehara E, Hokazono H, Hida M, Sasaki T, Yoshioka H, Matsuo N. GABA promotes
elastin synthesis and elastin fiber formation in normal human dermal fibroblasts
(HDFs)
37. Nakamura, H., et al. (2009). "Psychological stress-reducing effect of chocolate
enriched with γ-aminobutyric acid (GABA) in humans: assessment of stress using
heart rate variability and salivary chromogranin A." 60(sup5): 106-113.
38. H.C. Stanton, Mode of action of gamma-aminobutyric acid on the cardiovascular
system, Arch. Int. Pharmacodyn. 143 (1963) 195–204
39. R.A. Gillis, K.A. Yamada, J.A. DiMicco, D.J. Williford, S.A. Segai, P. Hamosh, W.P.
Norman, Central gamma-aminobutyric acid involvement in blood pressure control,
Fed. Proc. 43 (1984) 32–38.
40. Jones J. M., Reicks M., Adams J., Fulcher G., Weaver G., Kanter M., Marquart L. The
importance of promoting a whole grain food message. J. Am. Coll. Nutr.
2002;21:293–297. doi: 10.1080/07315724.2002.10719226.
41. Mi-Hye Kim,† Sung-Il Ahn,† Chan-Mook Lim, Jin-Woo Jhoo, and Gur-Yoo Kim,
Effects of Germinated Brown Rice Addition on the Flavor and Functionality of Yogurt
42. Rebecca Njeri Damaris 1,2, Zhongyuan Lin 1,2, Pingfang Yang 3,* and Dongli He,
The Rice Alpha-Amylase, Conserved Regulator of Seed Maturation and Germination
43. Abdul Rohman, Dwi Setyaningrum, Mirza Hapsari Sakti Titis Penggalih, Rice in
health and nutrition, International Food Research Journal 21(1): 13-24 (2014)
44. Kum J. S., Choi B. K., Lee H. Y., Park J. D., Park H. J. Physicochemical properties of
germinated brown rice. Korean J. Food Preserv. 2004;11:182–188.
45. Kang B. R., Park M. J., Lee H. S. Germination dependency of antioxidative activities
in brown rice. Korean J. Soc. Food Sci. Nutr. 2006;35:389–394. doi:
10.3746/jkfn.2006.35.4.389.
46. Kim K. J., Choi I. D., Kim D. S., Son J. R., Yang C. I., Chun J. Y. Physico-chemical
properties of giant embryo brown rice (Keunnunbyeo) Agric. Chem. Biotechnol.
2006;49:95–100.
47. Sasagawa A., Naiki Y., Nagashima S., Yamakura M., Yamazaki A., Yamada A.
Process for producing brown rice with increased accumulation of GABA using hignpressure treatment and properties of GABA-increased brown rice.
48. D. Karladeea,b, S. Suriyonga, γ-Aminobutyric acid (GABA) content in different
varieties of brown rice during germination
49. Gustavo Santoyoa, Gabriel Moreno-Hagelsiebb, Ma.del Carmen Orozco-Mosquedac,
BernardR. Glick, Plant growth-promoting bacterial endophytes, Microbiological
Research 183(2016)92-99
50. Shyam L. Kandel, Pierre M. Joubert, and Sharon L. Doty, Bacterial Endophyte
Colonization and Distribution within Plants Microorganisms. 2017 Dec; 5(4): 77
PMID: 29186821
51. Faegheh Etminani and Behrouz Harighi, Isolation and Identification of Endophytic
Bacteria with Plant Growth Promoting Activity and Biocontrol Potential from Wild
Pistachio Trees, Plant Pathol J. 2018 Jun; 34(3): 208–217.
52. Shine Km, Jamal M Khaled, Rejiniemon Thankappan Sarasam, Naiyf Sultan Alharbi
In-vitro antibacterial, antifungal, antioxidant and functional properties of Bacillus
amyloliquefaciens
53. Lassaad Belbahri, Ali Chenari Bouket, Imen Rekik, Faizah N. Alenezi, Armelle
Vallat, Lenka Luptakova, Eva Petrovova, Tomasz Oszako, Semcheddine Cherrad,
Sébastien Vacher, and Mostafa E. Rateb, Comparative Genomics of Bacillus
amyloliquefaciens Strains Reveals a Core Genome with Traits for Habitat Adaptation
and a Secondary Metabolites Rich Accessory Genome, PMID: 28824571
54. Nan Zhang, Dongqing Yang, Joshua R. A. Kendall, Rainer Borriss, Irina S.
Druzhinina, Christian P. Kubicek, Qirong Shen, and Ruifu Zhang, Comparative
Genomic Analysis of Bacillus amyloliquefaciens and Bacillus subtilis Reveals
Evolutional Traits for Adaptation to Plant-Associated Habitats
55. Giovanni Vinci, Vincenza Cozzolino, Pierluigi Mazzei, Hiarhi Monda, Davide Savy,
Marios Drosos, Alessandro Piccolo, Effects of Bacillus amyloliquefaciens and
different phosphorus sources on Maize plants as revealed by NMR and GC-MS based
metabolomics
56. Tiziano Baroni, Catia Bellucci, Cinzia Lilli, Furio Pezzetti, Francesco Carinci, Ennio
Becchetti, Paolo Carinci, Giordano Stabellini, Mario Calvitti, Eleonora Lumare, and
Maria Bodo, Retinoic Acid, GABA-ergic, and TGF-β Signaling Systems Are Involved
in Human Cleft Palate Fibroblast Phenotype
57. Neetu Tyagi, David Lominadze, William Gillespie, Karni S. Moshal, Utpal Sen,
Dorothea S. Rosenberger, Mesia Steed, and Suresh C. Tyagi, Differential expression
of γ-aminobutyric acid receptor A (GABAA) and effects of homocysteine Clin Chem
Lab Med. 2007; 45(12): 1777–1784. PMID: 17990949

指導教授

黃俊銘(Chun-Ming Huang)

審核日期

2019-7-12

推文