可調控式褐藻酸/聚己內酯複合傷口敷料

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姓名

李坪駿(Ping-Jyun Li) 查詢紙本館藏

畢業系所

化學工程與材料工程學系

論文名稱

可調控式褐藻酸/聚己內酯複合傷口敷料
(The Development of Tunable Alginate/Polycaprolactone Composite Wound Dressings)

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至系統瀏覽論文 ( 永不開放)

摘要(中)

複雜性的創傷如慢性傷口，因癒合緩慢，若持續未完善處理，一旦遭受細菌感染，就可能造成蜂窩性組織炎，更可能引發敗血症，因此需開發多功能的傷口敷料來因應其狀況，本研究選用褐藻酸鈉與聚己內酯為靜電紡絲的高分子材料，褐藻酸鈉具親水性可為傷口提供潮濕環境，聚己內酯則因其有良好的生物相容性以促進細胞貼附，除此之外，我們將奈米銀引入到聚己內酯纖維中使其抑制細菌的生長，以達到抗菌效果，並藉由將血小板衍生生長因子（platelet-derived growth factor B，PDGFB）與聚乙烯亞胺（PEI）結合為帶正電奈米顆粒，使其可與帶負電的褐藻酸鈉纖維做結合，再藉由原位轉染的方式使細胞可以表達PDGFB，最後藉由褐藻酸鈉與聚己內酯纖維各自不同的特性來調整不同比例的褐藻酸鈉與聚己內酯纖維，以達到擁有多功能效果的傷口敷料。

摘要(英)

Wound healing is an important issue in clinical situation. For example, chronic wounds are slow to heal. The prolonged inflammatory phase in chronic wounds may result in not only an immature granulation tissue but also a parallel reduction of wound tensile strength, which always eventually cause amputations. Therefore, it is necessary to develop a multifunctional wound dressing to promote tissue regeneration. In this study, alginate and polycaprolactone (PCL) fibers are investigated as materials for wound dressing preparation. Alginate is a hydrophilic material and can provide a moist environment in wound site. Polycaprolactone has good biocompatibility to promote cell adhesion. In addition, we introduced the silver nanoparticles into polycaprolactone fibers to inhibit the growth of bacteria. To promote tissue regeneration, plasmid DNA encoding platelet-derived growth factor B (PDGFB) was complexed with polyethyleneimine (PEI) as positively charged nanoparticles to adsorb on negatively charged sodium alginate fibers. Therefore, cells contact fibers can thus be transfected to express PDGFB. Through coelectrospinning, alginate and PCL were prepared as composite fibers, and their properties can be manipulated by adjusting fiber ratios to achieve a versatile wound dressing.

關鍵字(中)

★ 電紡絲
★ 褐藻酸
★ 聚己內酯

關鍵字(英)

論文目次

摘要 v
Abstract vi
致謝 vii
目錄 viii
圖目錄 xi
表目錄 xiv
第一章緒論 1
1-1研究背景 1
1-2研究目的 3
第二章文獻回顧 4
2-1電紡絲 4
2-1-1電紡絲簡介與原理 4
2-1-2電紡絲於傷口敷料上之應用 6
2-1-3複合電紡絲 8
2-2褐藻酸鈉 10
2-2-1褐藻酸鈉之簡介 10
2-2-2褐藻酸鈉之性質 11
2-2-3褐藻酸鈉於傷口敷料上之應用 13
2-3聚己內酯 15
2-3-1聚己內酯之簡介 15
2-3-2聚己內酯之性質 16
2-3-3聚己內酯於傷口敷料上之應用 17
2-4慢性傷口 19
2-4-1糖尿病之傷口修復 19
2-4-2促進傷口癒合的生長因子 23
2-4-3血小板衍生生長因子(PDGF)於糖尿病傷口之應用 24
2-5奈米銀粒子 25
2-5-1奈米銀粒子之簡介 25
2-5-2奈米銀粒子於傷口敷料的應用 28
2-6基因治療 30
2-6-1基因載體 30
2-6-2基因治療之應用 31
第三章實驗材料與方法 34
3-1實驗材料 34
3-2實驗儀器 38
3-3實驗方法 40
3-3-1電紡絲溶液製備 40
3-3-2含奈米銀粒子之PCL纖維製備 42
3-3-3電紡絲纖維製備 43
3-3-4溶液配製 44
3-3-5 NIH 3T3細胞培養 47
3-3-6 SEM之樣本製備 50
3-3-7 ATR-FTIR樣本製備 51
3-3-8萬能拉伸試驗機樣本製備 51
3-3-9細胞存活率實驗(MTT assay) 52
3-3-10質體DNA(PDGFB)純化 54
3-3-11原位轉染實驗 55
3-3-12測量PDGFB釋放實驗 58
3-3-13抗菌實驗 60
3-3-14奈米銀檢測[27] 62
3-3-15傷口癒合動物實驗 63
第四章結果與討論 65
4-1褐藻酸鈉/聚己內酯/奈米銀之複合電紡絲性質 65
4-1-1電紡絲型態與直徑分布 65
4-1-2複合電紡絲之ATR-FTIR分析 68
4-1-3複合電紡絲之機械性質分析 71
4-1-4複合電紡絲之接觸角分析 73
4-2複合電紡絲之奈米銀釋放 74
4-3複合電紡絲之抗菌活性 76
4-3-1液態培養之抑菌率 76
4-3-2固態培養之抗菌活性 78
4-4複合電紡絲對細胞之影響 81
4-4-1細胞於複合纖維上培養之SEM圖 81
4-4-2細胞於複合纖維上培養之增生率 84
4-5於複合電紡絲上進行原位轉染PDGFB 86
4-5-1原位轉染之螢光影像 86
4-5-2原位轉染後分析PDGFB釋放與細胞增殖率 90
4-6傷口癒合動物實驗 93
第五章結論 98
第六章參考資料 100

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指導教授

胡威文

審核日期

2021-1-28

推文