模擬與真實微重力下小鼠骨細胞基因表現的生物資訊研究

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姓名

王娟瑜(Chuan Yu Wang) 查詢紙本館藏

畢業系所

生醫科學與工程學系

論文名稱

模擬與真實微重力下小鼠骨細胞基因表現的生物資訊研究
(Bioinformatics Analysis of Gene Expression in Mouse Bone Cells Under Simulated and Real Microgravity Conditions)

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至系統瀏覽論文 (2030-7-31以後開放)

摘要(中)

研究旨在探討模擬與真實太空環境下，小鼠骨相關細胞基因表現的差異及其對骨質代謝的影響。透過分析公開數據庫GEO及NASA OSDR的基因表現數據，並結合顯著差異基因分析、KEGG通路分析及GSEA分析，我們確認多條與骨質代謝、細胞增殖及環境應激相關的信號傳導路徑，如Ribosome、PI3K-Akt、HIF-1及細胞週期調控途徑等等。結果顯示微重力下顯著影響細胞環境，調控細胞氧化壓力、代謝途徑表現，進而骨質流失及相關生理變化。這些發現有助於理解長期太空飛行對骨骼健康的影響，並為未來骨質疏鬆症的預防及治療提供潛在價值。

摘要(英)

This study aims to investigate the differential gene expression and metabolism of bone-related cells in mice under simulated microgravity or spaceflight. By analyzing gene expression data from publicly available databases such as GEO and NASA OSDR, and incorporating differential gene analysis, KEGG pathway analysis, and GSEA analysis, we identified multiple signaling pathways associated with bone metabolism, cell proliferation, and environmental stress responses, including the PI3K-Akt, HIF-1, Ribosome and cell cycle regulation pathways. The results demonstrate that microgravity significantly affects the cellular environment, regulating oxidative stress and metabolic pathways, ultimately contributing to bone loss and related physiological changes. These findings enhance our understanding of the effects of long-term spaceflight on skeletal health and provide potential value for the prevention and treatment of osteoporosis in the future.

關鍵字(中)

★ 微重力
★ 骨細胞
★ 基因表現

關鍵字(英)

★ microgravity
★ bone cell
★ gene expression

論文目次

中文摘要 i
Abstract ii
致謝 iii
一、緒論 1
1-1 研究動機 1
1-2 背景 1
1-3 骨頭的重塑 6
二、研究材料與方法 8
2-1 研究材料 8
2-1-1 基因表達資料庫（Gene Expression Omnibus , GEO） 8
2-1-2 NASA Open Science Data Repository (OSDR) 8
2-1-3 KEGG 9
2-2 研究方法 10
2-2-1 資料前處理 10
2-2-2 表現量計算 11
2-2-3 分析方法 12
三、結果與討論 14
3-1 顯著差異基因分析 14
3-1-1 顯著上調基因的重疊 19
3-1-2 顯著下調基因的重疊 20
3-1-3 全部顯著基因的重疊 24
3-2 KEGG pathway分析 24
3-3 GSEA分析 39
四、討論 44
五、結論 47
六、參考文獻 48
七、附錄 53
7-1 程式碼1 53
7-2 程式碼2 54
7-3 程式碼3 54
7-4 程式碼4 56
7-5 程式碼5 58

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指導教授

許藝瓊(Yi Chiung Hsu)

審核日期

2025-1-13

推文