AFM Studies on Phospholipid Bilayers

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姓名

廖宜鴻(Yi-Hung Liao) 查詢紙本館藏

畢業系所

物理學系

論文名稱

(AFM Studies on Phospholipid Bilayers)

相關論文

★ 用氘核磁共振儀研究含高濃度麥角脂醇的DPPC人造膜之分子交交互作用	★ Fluorescence study of lipid membranes containing sterol
★ 含固醇的脂質雙層膜的形態及相行為的研究	★ The effects of composition and thermal history on the properties of supported lipid bilayers
★ The effect of sterol on the POPE/DPPC membranes	★ 麥角固醇對含膽固醇的脂雙層膜的影響
★ Deuterium NMR Study of the Effect of Stigmasterol on POPE Membranes	★ Deuterium NMR Study of the effect of 7- dehydrocholesterol on the POPE Membranes
★ 運用氘核磁共振儀研究POPC/cholesterol膜之物理性質	★ 模型細胞膜(含有相同碳鏈的PC/PE)存在或缺乏固醇類的物理性質
★ 運用氘核磁共振研究DPPC/POPE/sterol人造細胞膜之物理性質	★ Phase Behavior and Molecular Interactions of Membranes Containing Phosphatidylcholines and Sterol: A Deuterium NMR Study
★ The physical properties of phytosterol-containing lipid bilayers	★ An AFM Study on Supported Lipid Bilayers with and without Sterol
★ β-谷固醇對POPE膜物理特性的影響	★ 固醇結構對PC膜物理特性的影響

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摘要(中)

我們使用原子力顯微鏡研究室溫下DPPC，POPC，及POPC/DPPC膜的形貌。這些膜是以雲母片為支撐基板的脂質雙層 (supported lipid bilayers)。我們比較以旋轉塗佈方法 (spin coating) 及脂球融合方法 (vesicle fusion) 製作的脂質雙層。這兩種方法製造的脂質雙層有相似的厚度，但旋轉塗佈方法可得到較一致的脂質覆蓋率。POPC (一種低熔點的脂質，Tm = -7 oC) 膜因其液態特性而呈現較DPPC (一種高熔點的脂質，Tm = 40 oC) 膜均勻的形貌，以及較小的脂質雙層厚度。我們也得到POPC/DPPC混和脂膜的形貌與DPPC濃度的關係圖，其中我們觀察到脂質不互溶性 (lipid immiscibility)。對脂質雙層厚度分析的結果暗示“富含POPC區塊”（POPC-rich domains）與“富含DPPC區塊”（DPPC-rich domains）共存。加入愈多DPPC會增加較厚之脂質雙層（即富含DPPC區塊）的比例，因此造成POPC/DPPC混和脂膜有較大的脂質雙層平均厚度。

摘要(英)

We have studied the morphologies of DPPC, POPC, and POPC/DPPC membranes at room temperature using atomic force microscopy (AFM). The membranes were lipid bilayers supported on mica surfaces. Lipid bilayers prepared by spin coating and vesicle fusion were compared. Both methods produce bilayers of similar thickness. However, spin coating results in more uniform lipid coverage. Because of the fluid nature of the bilayers, POPC (a low-melting lipid with Tm of -7 oC) present a more homogeneous morphology and a smaller bilayer thickness than DPPC (a high-melting lipid with Tm of 40 oC). The morphologies of binary mixtures of POPC and DPPC were obtained as a function of DPPC concentration. Lipid immiscibility is observed. Analysis of bilayer thickness suggests coexistence of POPC-rich and DPPC-rich domains. Addition of DPPC increases the fraction of the thicker-bilayer (i.e. DPPC-rich) domains, thus increase the average bilayer thickness of POPC/DPPC bilayers.

關鍵字(中)

★ 磷脂雙層

關鍵字(英)

★ Hydration
★ Lipid bilayer
★ Vesicle fusion
★ Spin coating

論文目次

Chapter 1 Introduction 1
1.1 Structure of Lipid Membrane 2
1.2 Model Membrane 5
Chapter 2 Materials and Method 8
2.1 Vesicle Fusion Method 8
2.1.1 Materials for Vesicle Fusion Method 8
2.1.2 Multilamellar Vesicles (MLVs) 9
2.1.3 Large Unilamellar Vesicles (LUVs) 9
2.1.4 Supported lipid Membrane 9
2.2 Spin Coating Method 10
2.2.1 Materials for Spin Coating 10
2.2.2 Spin Coating 10
2.3 Atomic Force Microscopy 11
2.3.1 Working Principles of AFM 12
2.3.2 AFM Operation Modes 13
2.3.3 Atomic Force Microscope and Tips 14
2.4 Data Processing and Analysis 15
2.4.1 Data Processing 15
2.4.2 Data Analysis 15
Chapter 3 Results and Discussions 16
3.1 Comparison of DPPC Membrane by Spin Coating and Vesicle
Fusion 16
3.2 POPC Membranes by Spin Coating 19
3.3 Hydration of Spin-Coated POPC Membrane 21
3.4 POPC/DPPC Mixture Membranes 25
Chapter 4 Conclusions 30
References 32

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指導教授

薛雅薇(Ya-Wei Hsueh)

審核日期

2007-7-12

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