探討光照對Saccharomyces cerevisiae生產乙醇之影響

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黃俊凱(Chun-Kai Huang) 查詢紙本館藏

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化學工程與材料工程學系

論文名稱

探討光照對Saccharomyces cerevisiae生產乙醇之影響
(Effect of light on the production of ethanol by Saccharomyces cerevisiae in batch culture)

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摘要(中)

由於石油蘊藏量有限，且國際原油價格不斷上漲，研究開發替代能源的重要性日益彰顯，在眾多替代能源中，生質酒精頗受全球矚目。生質酒精是指由生物物質所製成之酒精，生質酒精與汽油混合之燃料即為生質酒精汽油，為可再生燃料，發展生質酒精汽油使用自產能源可創造就業機會、增加能源來源多元化，同時減少溫室氣體排放。
本篇論文的主要目的是在探討「光」這個環境因子對於S. cerevisiae生產酒精之影響。主要探討的有白光、紅光、藍光三種不同光源在不同光強度下對Saccharomyces cerevisiae在菌體生長及產物生產上所造成的效應，再利用兩階段照光實驗同時提升菌體及產物的產量，實驗結果發現不同光源都有其最適光照強度，超過了最適光照強度則會對Saccharomyces cerevisiae產生抑制的現象，其中白光及紅光為600 Lux、藍光為900 Lux，不同光源對Saccharomyces cerevisiae也有不同生理效應產生:紅光會促進菌體的生長、藍光會促使酵母菌將基質轉化成酒精、白光則是同時促進菌體及酒精的產量，最後藉由兩階段照光實驗調控出最佳製程，其酒精產量為92.5 g/l為避光時的1.3倍，比生長速率也提升2.1倍，而productivity則提升了1.9倍之多。
整體而言，照光對Saccharomyces cerevisiae是有正面的幫助，且不同波長的光源的確會對Saccharomyces cerevisiae有不同的效應。

摘要(英)

The depletion of fossil fuel reserves, the unstable petrol prices and increasing environmental and political pressures has increased industrial foucs toward alternative fuel sources, in many alternative energy source, bio-ethanol was an attentively energy source in world-wide. Bio-ethanol can be blended with gasoline namely ethanol fuel, develop bio-ethanol could increase energy source, reduce exhaust green-house gases.
This research was to confer the light factor on ethanol production by Saccharomyces cerevisiae. This research was use three different wavelength light : White light, Red light, Blue light in different light intensity affected S. cerevisiae on biomass and ethanol production. We found different wavelength light has different optimal light intensity, white light and red light was 600 Lux, blue light was 900 Lux, and if the light intensity excess optimal light intensity, S. cerevisiae was inhibited. This research also found different wavelength has different physiological effect: Red light stimulated S. cerevisiae biomass production, Blue light stimulated S. cerevisiae specific ethanol yield, White light stimulated both biomass production and specific ethanol yield. This research use two-stage process to make optimal fermentation condition. Use two-stage process could make ethanol concentration reach 92.5 g/l, specific growth rate reach 2.1 fold (contrast with dark), ethanol productivity reach 1.9 fold (contrast with dark).

關鍵字(中)

★ 酵母菌
★ 乙醇
★ 光

關鍵字(英)

★ Saccharomyces cerevisiae
★ ethanol
★ light

論文目次

摘要 I
目錄 II
圖目錄 VI
表目錄 X
第一章緒論 1
1-1 研究動機 1
1-2 研究目的 2
第二章文獻回顧 4
2-1 真菌 4
2-1.1真菌介紹 4
2-1.2真菌分類表 5
2-1.3酵母菌(Saccharomyces cerevisiae)之介紹 5
2-2 再生能源 9
2-2.1 再生能源之介紹 9
2-2.2 各類再生能源應用及發展 12
2-2.3 生質酒精 15
2-2.4 我國發展生質酒精之策略 19
2-3 影響發酵的物理化學因子 21
2-3.1培養基組成 21
2-3.2溫度 22
2-3.3 pH值 23
2-3.4 溶氧量 24
2-3.5 通氣量 24
2-3.6 光 25
2-3.6.1 光接收器 25
第三章實驗規劃、材料與方法 31
3-1 實驗規劃 31
3-2 實驗材料 32
3-2.1 實驗菌株 32
3-2.2實驗藥品 33
3-2.3實驗儀器與設備 34
3-3實驗方法 35
3-3.1 菌種保存 35
3-3.2 培養基組成 35
3-3.3發酵槽培養 38
3-3.4 實驗分析方法 39
第四章實驗結果與討論 45
4-1 發酵槽培養實驗 45
4-2 發酵槽實驗之白光強度對Saccharomyces cerevisiae生產酒精發酵槽動力曲線圖之影響 46
4-2.1 白光強度對於Saccharomyces cerevisiae發酵時間的影響 51
4-2.2 白光強度對於Saccharomyces cerevisiae菌體生長的影響 51
4-2.3 白光強度對於Saccharomyces cerevisiae產物生產的影響 54
4-3 發酵槽實驗之紅光及藍光強度對Saccharomyces cerevisiae 生產酒精發酵槽動力曲線圖之影響 58
4-3.1 紅光及藍光強度對於Saccharomyces cerevisiae發酵時間的影響 64
4-3.2 紅光及藍光強度對於Saccharomyces cerevisiae菌體生長的影響 64
4-3.3 紅光及藍光強度對於Saccharomyces cerevisiae產物生產的影響 67
4-4 發酵槽實驗之兩階段照光對Saccharomyces cerevisiae生產酒精發酵槽動力曲線圖之影響 70
4-4.1兩階段照光對於Saccharomyces cerevisiae發酵時間的影響 73
4-4.2兩階段照光對於Saccharomyces cerevisiae菌體生長的影響 73
4-4.3 兩階段照光對於Saccharomyces cerevisiae產物生產的影響 75
第五章結論與相關建議 77
5-1結論 77
5-2建議: 78
第六章參考文獻 80

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指導教授

徐敬衡(Chin-hang Shu)

審核日期

2008-7-17

推文