奈米碳管電容式生物感測器對於DNA的檢測

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姓名

劉鎮宇(Chen-Yu Liu) 查詢紙本館藏

畢業系所

機械工程學系

論文名稱

奈米碳管電容式生物感測器對於DNA的檢測
(The capacitor-based carbon nanotube biosensor for DNA detection.)

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摘要(中)

本論文主要探討奈米碳管對於去氧核醣核酸生物感測器的研究，元件以電容器結構的作為量測重點，目的在於提升感應度與靈敏性。
實驗可分為三部分，首先我們將DNA以化學處理方式固定於氮化矽基板上並進行雜交實驗，來做為日後的控制實驗組；接著在氮化矽基板上成長奈米碳管，然後將DNA固定於上，一樣進行雜交實驗；最後再將基板材料更換為高介電常數材料五氧化二鉭，同樣地重複以上實驗。
在結果的量測與分析中，則是著重於此電容式生物感應器的電容電壓特性曲線上，因載子在空乏狀態時會受DNA自身帶負電的影響，而使圖形產生了曲線偏移現象，因此我們可用來確認DNA的固定化與雜交，另外利用公式後可進一步推算DNA固定化後密度。

摘要(英)

This study focused on the carbon nanotubes biosensors for deoxyribonucleic acid. We measured the capacitor structure as key aim then improve the sensitivity and selectively.
The experiment could be divided into three parts. First part, we tried the DNA immobilization and hybridization experiments on silicon nitride which was the control sample in the all experiments. Then we synthesized the carbon nanotubes on the bare silicon nitride and repeated the DNA immobilization and hybridization again. In the future, we plan that try to use the high dielectric materials instead of the silicon nitride and repeat both the DNA immobilization and hybridization again.
In the experiment results and analysis, we focused on the capacitance-voltage curve of the capacitor-based biosensor. The curve will perform the voltage-shift due to DNA molecules are negatively charged. Therefore, we could check both the immobilization and hybridization then calculated the density of DNA after hybridization.

關鍵字(中)

★ 電容
★ 氮化矽
★ 去氧核醣核酸
★ 奈米碳管
★ 生物感測器

關鍵字(英)

★ Carbon nanotubes
★ Capacitance
★ biosensor
★ DNA
★ Silicon nitride

論文目次

中文摘要 i
英文摘要 v
誌謝 vi
目錄 vi
圖目錄 x
表目錄 xv
符號說明 xvi
第一章緒論 1
1-1 介紹 1
1-2 文獻回顧 2
1-3 實驗動機 6
第二章奈米碳管的介紹 7
2-1 奈米碳管的起源 8
2-2成長機制 9
2-3 合成奈米碳管的方法 11
2-4結構及其特性 16
2-5 應用 20
第三章實驗方法與設備 24
3-1 實驗流程 25
3-2 試片製備 26
3-2-1 Control sample製備 27
3-2-2 奈米碳管試片製備 28
3-2-3 High-k材料試片製備 30
3-3 實驗步驟 32
3-3-1實驗步驟方法 34
3-3-2參考電極之製備 34
3-3-3 DNA定量分析 36
3-4 製程設備與量測設備介紹 39
3-4-1 製程設備介紹 39
3-4-2 量測設備介紹 43
3-5 元件設計與光罩介紹 47
3-6 奈米碳管品質的分析 52
第四章結果與討論 54
4-1以氮化矽為基板之控制試片 55
4-1-1 接觸角量測系統 55
4-1-2 AFM原子力探針顯微鏡 59
4-1-3 FTIR 62
4-1-4 奈米元件量測系統－電容電壓特性分析 63
4-2添加奈米碳管的生物感測器 76
4-2-1 奈米碳管的結構與品質（SEM、TEM與Raman） 76
4-2-2 接觸角量測系統 80
4-2-3 Raman光譜分析 83
4-2-4 電容電壓特性 84
4-3 高介電薄膜生物感測器 90
4-3-1 X-ray diffraction 的分析 91
第五章總結 92
參考文獻 93

參考文獻

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指導教授

鄔蜀威、丁志華、黃豐元
(Shu-Wei Wu、Jyh-Hua Ting、Fuang-Yuan Huang)

審核日期

2010-7-15

推文