Application of Steric-Mass Action model to triblock copolymer as a displacer in ion-exchange displacement chromatography

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Title:	Application of Steric-Mass Action model to triblock copolymer as a displacer in ion-exchange displacement chromatography
Authors:	Chen,WY;Dai,KC;Ho,KC;Lin,FY;Huang,HM
Contributors:	化學工程與材料工程學系
Keywords:	INDUCED SALT GRADIENTS;METHACRYLIC POLYAMPHOLYTES;DEXTRAN SULFATE;ABC TRIBLOCK;PROTEINS;AFFINITY
Date:	1999
Issue Date:	2010-06-29 17:28:29 (UTC+8)
Publisher:	中央大學
Abstract:	This study explores the feasibility of using triblock copolymer polyampholytes as a displacer to separate beta-lactoglobulin A and B isoforms by anion exchange displacement chromatography. The displacers used herein are methacrylic polyampholytes: A(8)M(12)B(12), A(16)M(12)B(12) and A(16)M(24)B(24), where A is methacrylic acid, B is 2-(dimethylamino)ethyl methacrylate, and M is methyl methacrylate. In addition, the steric factor, characteristic charges and binding equilibrium constant with the ion exchanger gel of the triblock copolymer are measured and discussed with the structure and solution behavior of the polyampholytes. With the Steric-Mass Action (SMA) model of ion-exchange displacement chromatography, the elution and displacement line can be constructed for the system. Moreover, the behavior of elution and displacement line is qualitatively discussed and successfully proven.
Relation:	JOURNAL OF THE CHINESE INSTITUTE OF CHEMICAL ENGINEERS
Appears in Collections:	[National Central University Department of Chemical & Materials Engineering] journal & Dissertation

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