溫度及鈣動力學對離體心臟心率之影響; The effects of temperature and calcium dynamics on cardiac interbeat intervals

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题名:	溫度及鈣動力學對離體心臟心率之影響;The effects of temperature and calcium dynamics on cardiac interbeat intervals
作者:	涂筱雯;Hsiao-wen Tu
贡献者:	生物物理研究所
关键词:	變異性;心率;鈣;溫度;心臟;Interbeat interval;Calcium;Temperature;Heart;variability
日期:	2010-07-30
上传时间:	2010-12-08 14:18:25 (UTC+8)
出版者:	國立中央大學
摘要:	心臟心率是靠竇房節(SA node)控制,竇房節藉由細胞及細胞間的偶合產生同步之跳動。在離體老鼠全心臟的灌流系統中，我們利用藥物調控鈣離子濃度以研究鈣動力對竇房節心率之影響，我們亦探討溫度的影響。心臟細胞培養系統可用來模擬竇房節，在此系統下我們討論溫度及纖維母細胞濃度的效應。心率的長條統計圖可以顯示平均的心率及心率變異性隨著溫度增加而上升。並且他們之間呈現冪次定理關係(power low relation)。心率之Poincare map和angle map也顯示在高溫時心率是相對規律的。溫度也許會影響細胞間偶合的強度導致 AV delay隨著溫度下降而變長。我們在心臟細胞培養系統觀察到和離體心臟相似的結果，心率和傳導速度隨著溫上升而上升。在不同溫度下心率和傳導速率呈現線性關係，由此可看出波長是固定的。纖維母細胞會隨著培養天數而增生進而降低心率及傳導速度。 Cardiac interbeat interval (IBI) is regulated by sinoatrial (SA) node. The information of contraction provided by SA node propagates through inter-cell coupling; leading to synchronous contrac- tion. To investigate the effect of calcium dynamics on the beat rate of SA node, the heart of rat in a Langendorff system is cannulated and perfused with different chemicals which tune the calcium dynamics. As for the temperature effect, we tune the temperature in both the whole heart system and the primary cardiac co-culture of rat. Temperature is used as a parameter to change system properties (intrinsic frequency and coupling strength) on heart rate. Co-cultures of cardiac myocytes and fibroblasts are used to model the SA node. In the whole heart system, the histogram of IBI shows that both the average IBI and the IBI variability decrease with increasing temperature. The IBI variability and average IBI exhibit power law relation. The Poincare map of IBIs and the angle map also show more regular IBI at higher temperature. The longer atrioventricular (AV) delay with decreasing temperature may be caused by the temperature-dependent coupling. In the culture system, the beat frequency (BF) and conduction velocity (CV) increase with temperature; similar to the results of whole heart experiment. The linear relation between BF and CV suggests indicates a constant wavelength. The higher fibroblast density in cultures with longer culture time decreases both the BF and CV.
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