設計一網印電極藉由電阻抗感測偵察細胞行為;Screen-printed electrode design for cell behaviors detection by electric cell-substrate impedance sensing

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题名:	設計一網印電極藉由電阻抗感測偵察細胞行為;Screen-printed electrode design for cell behaviors detection by electric cell-substrate impedance sensing
作者:	林盈瑞;Lin,Ying-Jui
贡献者:	電機工程學系
关键词:	指叉電極;掃描式電子顯微鏡;細胞與基底間電性檢測法;靈敏度;electric cell-substrate impedance sensing;interdigitated electrode;scanning electron microscope;sensitivity
日期:	2013-01-28
上传时间:	2013-03-25 16:18:43 (UTC+8)
出版者:	國立中央大學
摘要:	本研究設計一拋棄式銀電極作為量測工具，以細胞與基底間電性檢測法(ECIS)為分析方法，針對老鼠纖維母細胞L929進行即時監控，以及小鼠巨噬細胞RAW264.7發炎行為來驗證電極設計原理。指叉電極由網印方式製造， LCR電表確認其基本電氣特性。在細胞阻抗實驗量測中，自製指叉電極能準確監控細胞L929在電極表面之行為，當細胞貼附在一對電極表面狀態十二小時下中頻歸一阻抗變化(NIC)最高可達800%。實驗過程中搭配掃描式電子顯微鏡技術(SEM)驗證細胞型態與阻抗之關係。為了驗證電極設計原理，本研究利用小鼠巨噬細胞進行發炎反應，當電極尺寸貼近細胞尺寸狀態時可有效提升感測器整體之阻抗靈敏度。上述實驗證明出當細胞貼附在電極表面時，電極對數為一對，而間距以及寬度為50μm之電極規格具有最高阻抗靈敏度，可減少電極表面之電場穿越長度使電場強度侷限在電極表面。除此之外為了確認生物相容性此網印電極利用MTT分析方法來驗證此電極適合細胞貼附。In this study, aimed to monitor L929 cell behaviors in real time, a disposable silver electrode was designed and incorporated in an electric cell-substrate impedance sensing analysis. Inflammation of cell RAW264.7 was developed to verify the electrode design theory. The electric characteristic of the electrode was measured with an LCR meter. This home-made interdigitated electrode was fabricated by screen printing technique. In the cell impedance measurement experiment, the normalized impedance change (NIC) of cell attaching on the one-paired electrode could be 800%, revealing the potentiality of our home-made electrodes to monitor cell behaviors on the electrode. The relationship between the measured impedance and the cell morphology on the electrode would be explained by scanning electron microscope (SEM) technique. Inflammation of cell RAW264.7 was developed to verify the electrode design theory. When the cell size was commensurate with the electrode size, the sensitivity of our sensor would be increased. Our experiment results showed that the one-paired electrode, with 50 μm spacing and width, had the highest sensitivity, supposedly because of more concentrated electric field distribution surfacing the substrate and electrode. Besides, MTT assays verified the compatibility of our home-made electrodes.
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