本研究是利用疏水性離子液體[Hmim][PF6]對Clostridium acetobutylicum進行萃取式發酵，以降低發酵液中丁醇濃度來改善產物抑制效應問題，並且搭配活性碳吸附來解決離子液體所造成的毒性問題。以1:1的比例進行萃取發酵時，可降低發酵液中丁醇濃度2.2g/L，而離子液體可經由水洗後真空濃縮來重複利用，但菌體受到毒性的影響則無法繼續生產丁醇。新鮮培養液若接觸離子液體後，用於菌種培養時菌體完全無法生長；但若由活性碳以1 : 10 (w/v) 吸附5分鐘後，用於培養菌體可達到控制組約70 %的丁醇產量，且發酵液經由活性碳吸附亦可降低發酵液中丁醇濃度。但此操作條件應用於萃取發酵後雖移除了2.99 g/L丁醇，但菌體所受的毒性抑制效果仍未見起色。因此未來應尋找較佳的操作條件以達到提昇產量的效果。 In this research, the hydrophobic ionic liquid, [Hmim][PF6] was applied to the extractive fermentation of Clostridium acetobutylicum to remove butanol from the broth. The toxic ionic liquid remained in the broth was adsorbed by activated carbon. During the extraction operation, 2.2 g/L of butanol was removed from the broth. The production of butanol was stopped after the extraction due to the toxicity of residual ionic liquid. C. acetobutylicum cannot grow in the medium which had contact with the ionic liquid. After adsorption by 1 : 10 (w/v) activated carbon for 5 minutes, the production of butanol by C. acetobutylicum can reach 70% of the amount of control experiment . The fermentation after extraction and adsorption can remove 2.99 g/L of butanol in the broth. But the butanol production cannot be enhanced by current operation conditions. It means that more operation conditions should be discussed to find the optimal way to improve the butanol production.
