研究期間：10108~10207;We propose a novel droplet-based microfluidic system. The microdroplets were generated from two immerscible fluid streams and disperse in the microchannel. Compare with continuous microfluidic system, droplet-based microfluidic system provides lots of unique advantages and can be used in the biomedical or chemical reaction applications. In this research proposal, we propose using droplet-based microfluidic system to use in cell culture and mass spectrometry interfacing application. In cell culture application, the microdroplet chip can efficiently separate the continuous fluids into individual droplets. Each droplet represent as a single cell culturing chamber. Thus, cell can be cultured in a high-throughput format without cross-contamination issues. In mass spectrometry analysis application, the droplet size represent as interfacing resolution. Using droplet-based microfluidic chip, the continuous-phase analyte can be diced into extremely small microdroplets and inject into the mass spectrometry target plate for following mass spectrometry analysis. The droplet formation mechanism and the microchannel design will need to further explore. Therefore, in this proposal, we use numerical simulation analysis to design the microchannel and experimentally evaluate the microdroplet chip performance.