黑色素的奈米化大幅提升其用在保護細胞對抗活性氧壓力的效用;Dramatic Improvement of the Efficacy of Melanin on Protecting Cells against ROS Stress by Its Nanonization

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题名:	黑色素的奈米化大幅提升其用在保護細胞對抗活性氧壓力的效用;Dramatic Improvement of the Efficacy of Melanin on Protecting Cells against ROS Stress by Its Nanonization
作者:	陳思旻;Chen,Sih-min
贡献者:	生物物理研究所
关键词:	黑色素;奈米薄片;水分散性;活性氧;自由基;氧化壓力;急性輻射綜合症;melanin;nano-flake;water-dispersible;reactive oxygen species;free radical;oxidative stress;acute radiation syndrome
日期:	2014-08-28
上传时间:	2014-10-15 14:39:05 (UTC+8)
出版者:	國立中央大學
摘要:	目的：黑色素是已知具有清除自由基及活性氧的能力。本論文提出利用奈米化黑色素作為藥物來對抗內源性氧化壓力或外源性急性氧化壓力，例如：急性輻射綜合症。材料與方法：發展一個簡單的技術，利用機械式攪拌來進行黑色素奈米化，使黑色素具有奈米尺度大小與親水性。存活率測試使用視網膜色素上皮細胞，將其暴露在外源性H2O2壓力下，並以不同條件的黑色素及照光來處理。結果與討論：機械式攪拌可以有效奈米化黑色素。在視網膜色素上皮細胞暴露在外源性活性氧壓力下，奈米化黑色素相較於未處理的黑色素明顯有效提高存活率。這個結果在不照光時有更明顯的提升，有希望應用於靜脈給藥的體內試驗。;Aim: It is known that melanin has the ability of scavenging free radicals and reactive oxygen species (ROS). The authors propose to utilize nanonized melanin as medication against endogenous chronic oxidative stress or exogenous acute oxidative stress caused by, e.g., acute radiation exposure. Materials & Methods: A simple technique based on mechanical stir was developed for nanonization of melanin to produce nanometer-sized and water-dispersible melanin. The viabilities of cultured retinal pigment epithelium (RPE) cells exposed to exogenous H2O2 stress and treated with various conditions of melanin and irradiation were compared. Results & Conclusion: Melanin can be nanonized very effectively with simple mechanical stir. Nanonized melanin exhibited a much stronger effect than unprocessed melanin on raising the viability of cultured RPE cells under exogenous acute ROS stress. The effect was even more prominent without simultaneous light irradiation, promising for effective in vivo application to the whole body via intravenous administration.
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