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    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/68207

    Title: 磷酸根甲基化去氧核醣核酸:合成、定性及其應用之研究;Phosphate-Methylated DNA as Neutralized DNA (nDNA):Synthesis, Properties and Potential Applications
    Authors: 林耀能;Lin,Yao-neng
    Contributors: 化學工程與材料工程學系
    Keywords: 去氧核醣核酸;場效電晶體
    Date: 2015-08-21
    Issue Date: 2015-09-23 10:53:18 (UTC+8)
    Publisher: 國立中央大學
    Abstract: 近年來,由於生物技術的快速進展,人體中基因所表現、調控的功能,與其所提供人體當下的資訊也逐漸明朗。基因晶片是瞭解人體內的基因表現資訊很有用的工具,以非侵入性、大量、快速且低成本之檢測方式得到樣品中的DNA、RNA的表現資訊,可應用於早期的病症診斷,也可以針對治療效果進行監控及甚至提出進一步的治療方向。
    ;Intensive efforts have been achieved on the sequencing of whole human genome, which revealed the blueprint of what we are made of, but we do not yet know how it work without a guideline. DNA microarray is a useful tool for early diagnosis and prompt therapeutic intervention, however, low DNA probe recognition efficiency are usually affected by some specific sequences. To improve the sensitivity and specificity of DNA probe, we synthesized a novel neutral DNA (nDNA), which is a DNA analogue with the backbone phosphate groups changed by methylposphate groups. nDNA showed high affinity to hybridize with its complementary regular DNA and nDNA, even at low ionic strength buffer conditions. In particular, nDNA also improve the recognition efficiency for mismatch DNA sequences. In this study, we synthesized and characterized four nDNA nucleosides for nDNA synthesis. nDNAs were synthesized through the solid-phase synthesis and purified by reversed-phase chromatography. From the circular dichroism spectrum and gel electrophoresis we found that nDNA and its complementary DNA can form duplex, and this duplex shows higher Tm than regular DNA duplex. Finally, we took nDNA as probe on FET (field-effect transistor) sensor for gene detection, the results revealed that nDNA can enhance the recognition efficiency under low ionic strength condition.
    Appears in Collections:[化學工程與材料工程研究所] 博碩士論文

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