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|Title: ||組織工程用冷凍成型積層製造之固態水支撐結構生成研究;Generation Process of Support Structure by Water Phase Change for Frozen-Form Additive Manufacturing of Tissue Engineering|
|Authors: ||吳偉任;Wu, Wei-Jen|
|Keywords: ||組織工程支架;冷凍成型積層製造;噴霧閥;支撐結構;tissue engineering scaffold;frozen-form additive manufacturing;spray valve;support structure|
|Issue Date: ||2017-01-23 17:13:01 (UTC+8)|
;Additive manufacturing technology has the advantage in rapid customization, and it is suitable for the production of tissue engineering scaffolds with high porosity and complex shape. This study is focusing on frozen-form additive manufacturing. However, it was difficult to produce the scaffolds with complex shape and porous structure, because its support structure could not be removed completely. This study developed a support process to rapidly generate the support structure and was removed completely.
Firs, This study improved the uniform cryogenic device module and added environmental control module into frozen-form additive manufacturing to enhance the freezing capacity and produce large complex shape scaffolds and rapidly generate support structure. The retaining steel and the copper tube were combined to become the cooling enclosure, which reduced energy loss. The lowest temperature of the working plate became -50°C from -30°C, and the time for the temperature to reach -30°C was reduced from 150 minutes to 20 minutes. Additionally, the support structure was generated by water phase change. Liquid was sprayed on the working plate through the spray valve, and it was frozen into solid to generate the support structure. Finally, the support structure was completely removed by vaporization. According to the literatures and the result of thermal imaging camera, 10wt% ethanol solution was selected as the support material and deposited onto 75 55mm area in 35 seconds.
In order to verify the effectiveness of the support process, PU/PEO was used as the scaffold material to produce cylindrical scaffolds. The cell viability testing was performed using MTT-assay. The results showed that the number of cells grew 1.8 times in 24 hours. The storage module of the scaffolds was lower with the support process, because of swelling effect, but it had a little effect on the elastic recovery. In the experimental observation, the scaffolds restored origin shape in 2 sec after extrusion. Furthermore, the micro/nano network structure of the scaffold was generated between strand and strand after the support process. The micro/nano network structure was detected by nuclear magnetic resonance spectroscopy, and the results showed that it was the material of the scaffold. Finally, the dual inverted T-type inner channels scaffold and the Y-type scaffold were produced in this study. From the above, this study verity the approach to produce large complex shape and porous structure tissue engineering scaffolds.
|Appears in Collections:||[機械工程研究所] 博碩士論文|
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