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    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/72885

    Title: 綠茶表沒食子酸酯型兒茶素酸酯在3T3-L1脂肪細胞分化過程中調節FoxO家族基因的表現;Green tea epigallocatechin gallate regulates the expression of the FoxO family during differentiation of 3T3-L1 fat cells
    Authors: 陳毅;Chen, Yi
    Contributors: 生命科學系
    Keywords: 脂肪細胞;兒茶素;細胞分化;FoxO;EGCG;adipocyte;differentiation;ATF-3
    Date: 2017-01-25
    Issue Date: 2017-05-05 17:13:08 (UTC+8)
    Publisher: 國立中央大學
    Abstract: Forkhead box class O (FoxO)家族基因包含有FoxO1、FoxO3、FoxO4和FoxO6四種,過去有許多文獻分別指出各FoxO基因在細胞內調控了包括分化、葡萄醣代謝、DNA修復和細胞凋亡在內的許多功能。在本實驗中我們使用了3T3-L1前脂肪細胞分化為脂肪細胞的系統,透過處理Dexamethasone、Insulin和3-isobutyl-1-methylxanthine等3種藥物所組成之分化劑,促使細胞進行為期14天的分化。在分化的過程中,細胞的數目有明顯的增加,而細胞內會有三酸甘油脂的堆積。在我們的實驗中發現,相較於沒有處理EGCG的組別,在分化期間處理EGCG會使細胞內油滴的數量減少。當沒有處理EGCG時,細胞的FoxO1、FoxO3、FoxO4和FoxO6的mRNA表現量在分化過程的第2~6天之間會有增加的趨勢,而在第6~14天之間的表現量則會有降低的趨勢。在14天的分化期間,EGCG會大幅降低FoxO1、FoxO3、FoxO4和FoxO6的mRNA表現量。此外ATF-3的mRNA表現量則會有增加的趨勢,其表現量並不會受到EGCG的影響。這些實驗結果顯示EGCG會透過調節FoxO的表現量去影響3T3-L1前脂肪細胞的分化。
    ;The forkhead box class O (FoxO) family of mammals contained 4 members: FoxO1, FoxO3, FoxO4 and FoxO6. Previous studies have revealed that FoxO genes regulate many functions in cells, including differentiation, glucose metabolism, DNA repair and apoptosis. In this study, we used the preadipocyte-adipocyte differentiation system of 3T3-L1 cells, induced by dexamethasone, insulin and 3-isobutyl-1-methylxanthine for a period of 14 days. During the differentiation, the cell number increased, and the TG droplet increased in cells. Our study also found that the TG droplet decreased when 3T3-L1 cells were treated EGCG, compared to control. In the absence of EGCG, FoxO1, FoxO3, FoxO4, and FoxO6 tended to increase their mRNA expressions from Day 2 to Day 6 of differentiation and decrease from Day 6 to Day 14 of differentiation. EGCG, at 100 μM, but not at 25 or 50 μM, significantly decreased levels of FoxO1, FoxO3, FoxO4, and FoxO6 mRNAs during a 14-day period of differentiation. Moreover, activating transcription factor (ATF)-3 tended to increase its mRNA expression and was not altered by EGCG treatment. These data suggest that EGCG regulates 3T3-L1 adipocyte differentiation through modulations of FoxO but not ATF-3 levels.
    Appears in Collections:[生命科學研究所 ] 博碩士論文

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