| 摘要: | 重金屬污染水體受矚目已久，銅(Cu)雖為微量元素，被攝入至生物體中卻會造成肝、腎及神經系統等疾病，對人體及環境皆有嚴重危害性，因此，開發快速及精確之Cu量測技術相當具有潛力。本研究利用電鍍沉積法製備金奈米粒子/幾丁聚醣-單壁奈米碳管複合材料修飾玻璃碳電極(Au/Chitosan-SWCNT/GCE)對水中Cu(II)進行伏安法分析。由於金奈米粒子具有高親和力和電催化活性及幾丁聚醣良好的成膜能力與黏附性可有效提高單壁奈米碳管的反應特性及分析靈敏度。研究結果顯示電極最佳製備參數為:電鍍電位-0.6 V、電鍍濃度0.5 mM、電鍍時間300秒、幾丁聚醣濃度0.1% ;而Cu(II)分析最佳掃描條件為:電解液0.1 M、掃描速率0.2Vs-1，偵測極限為3.24 ppb。此外，本研究亦添加其他重金屬Pb(II)、Cr(III)、Cr(VI)、As(III)、As(V)、Zn(II)、Cd(II)和Fe(III)做干擾試驗，探討添加不同金屬對Cu(II) 伏安法分析之影響，結果顯示除了Pb(II)和As(III)，其他金屬與Cu(II)同時存在於水中並不會影響Cu(II)的峰電流值(誤差值皆小於10%)，而Pb(II)和As(III)則會使Cu(II)之峰電流值上升，干擾伏安法量測Cu(II)之準確性，推測原因為Pb(II)和As(III)會與幾丁聚醣進行反應，使幾丁聚醣除捕捉Cu(II)外，亦同時捕捉Pb(II)或As(III)造成峰電流值提升。本研究之修飾電極對於檢測銅具有製備快速、操作簡單、高靈敏度等優點，可應用於環境監測，進而達到迅速掌握水質狀況與偵測污染排放之效果。;Heavy metal pollution in aquatic environment has drawn great attention for years. Though copper (Cu) is a trace element, also has toxic effects on human health and environment. Therefore, developing a fast and accurate sensor for Cu analysis is important. In this study, the gold nanoparticles/chitosan-single walled nanotubes modified glassy carbon electrode (Au/chitosan-SWCNT/GCE) was prepared for detection of Cu(II). The liner-sweep voltammetry method (LSV) was used to analyze Cu(II) and the gold nanoparticles were deposited on the single-walled nanotube through electroplating for enhance the reactivity of electrode. The optimum electroplating conditions of electrode preparation were -0.6 V, 0.5 mM HAuCl4, 300 s, and 0.1% chitosan. Besides, the optimum conditions of scanning Cu(II) were 0.1 M HCl (supporting electrolyte) with scan rate of 0.2 Vs-1. The range of scan potential was from 1.0 to -0.6 V and the detection limit of Cu(II) was 3.24 ppb. The interferences of Pb(II), Cr(III), Cr(VI), As(III), As(V), Zn(II), Cd(II), and Fe(III) ions were also investigated. The results indicated that, except Pb(II) and As(III) , the metal ions used in this work had no effects on the peak current of Cu (error less than 10%). However, Pb(II) and As(III) increased the peak current of Cu, it might be because that both metals could also chelate with chitosan. The Au/chitosan-SWCNT/GCE prepared in this study presents lots of attractive features for Cu(II) detection, such as simple preparation method, high sensitivity, good reproducibility, low detection limit, and quick current response. Thus, the modified electrode may be an available sensor for Cu(II) analysis via LSV and applied in environmental pollutant monitoring. |
