慢性傷口癒合緩慢，可能會造成很多風險與生活上的不便，因此本研究欲開發多功能傷口敷料以促進組織再生。我們以雙噴射電紡絲系統將褐藻酸鈉與聚己內酯（poly (ε-caprolactone)，PCL）混紡成多功能複合納米纖維。其中親水性的褐藻酸鈉纖維具高吸收性，可以在傷口部位提供潮濕環境，PCL則可增加機械強度並促進細胞貼附。將奈米銀導入PCL纖維中使其可以持續抑制微生物的生長。由於血小板衍生生長因子（platelet-derived growth factor B，PDGFB）是嗜中性粒細胞的化學引誘物並且可以誘導成纖維細胞的增殖和分化，進而促進傷口修復，因此我們將PDGFB質粒DNA與聚乙烯亞胺（PEI）所複合成的正電性奈米顆粒通過靜電相互作用吸附於複合纖維中的陰離子褐藻酸鈉纖維上。當傷口細胞貼附到複合纖維時將被原位轉染，而這些被轉染的細胞將可連續表達PDGFB。此外，褐藻酸鈉纖維中的鈣離子通過離子交換釋放到傷口部位以加速止血。這種全面的敷料為慢性傷口的治療提供了理想的解決方案。;Chronic wounds may retard the healing process to cause many risks. Therefore, it is essential to develop a multifunctional wound dressing to promote tissue regeneration. To fabricate a versatile composite nanofibrous matrix, sodium alginate and poly (ε-caprolactone) (PCL) were coelectrospun as composite nanofibers using a dual jet system. Hydrophilic alginate fibers may provide a moist environment in wound sites. In addition, PCL were applied to increase mechanical strength and cell adhesion. Silver nanoparticles were embedded in PCL fibers for long-term release to inhibit the growth of microorganism. Plasmid DNA encoding platelet-derived growth factor B(PDGFB) was delivered from composite fibers because this growth factor is a chemoattractant for neutrophils and can induce the proliferation and differentiation of fibroblasts. These PDGFB plasmids were complexed with polyethylenimine (PEI) to form cationic nanoparticles which may thus be adsorbed onto anionic alginate fibers through electrostatic interaction. As wound cells adhered to composite fibers, they can be in situ transfected to continuously express PDGFB. Moreover, calcium ions in alginate fibers were released to wound sites through ion exchange to accelerate hemostasis. This comprehensive dressing provides an ideal solution to heal chronic wounds.
